PUBLICATION
Characterization of a zebrafish/mouse somatic cell hybrid panel
- Authors
- Chevrette, M., Joly, L., Tellis, P., Knapik, E.W., Miles, J., Fishman, M., and Ekker, M.
- ID
- ZDB-PUB-000327-1
- Date
- 2000
- Source
- Genomics 64(1): 119-26 (Journal)
- Registered Authors
- Chevrette, Mario, Ekker, Marc, Fishman, Mark C., Joly, Lucille, Knapik, Ela W., Miles, Jennifer, Tellis, Patricia
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Genetic Linkage
- Genetic Markers
- Hybrid Cells
- In Situ Hybridization, Fluorescence
- Mice
- Microsatellite Repeats
- Polymerase Chain Reaction
- Zebrafish/genetics*
- PubMed
- 10708527 Full text @ Genomics
Citation
Chevrette, M., Joly, L., Tellis, P., Knapik, E.W., Miles, J., Fishman, M., and Ekker, M. (2000) Characterization of a zebrafish/mouse somatic cell hybrid panel. Genomics. 64(1):119-26.
Abstract
We have characterized a collection of zebrafish/mouse somatic cell hybrids with 211 genes and markers chosen from the 25 zebrafish linkage groups. Most of the zebrafish genome is represented in this collection with 88% of genes/markers present in at least one hybrid cell line. Although most hybrids contain chromosomal fragments, there are a few instances where a complete or nearly complete zebrafish chromosome has been maintained in a mouse background, based on multiple markers covering the entire chromosome. In addition to their use in mapping studies, this collection of somatic cell hybrids should constitute an important tool as a source of specific chromosome fragments and for assessing the function of genome regions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping