PUBLICATION
Cooperative roles of Bozozok/Dharma and Nodal-related proteins in the formation of the dorsal organizer in zebrafish
- Authors
- Shimizu, T., Yamanaka, Y., Ryu, S., Hashimoto, H., Yabe, T., Hirata, T., Bae, Y., Hibi, M., and Hirano, T.
- ID
- ZDB-PUB-000309-27
- Date
- 2000
- Source
- Mechanisms of Development 91(1-2): 293-303 (Journal)
- Registered Authors
- Bae, Young Ki, Hashimoto, Hisashi, Hibi, Masahiko, Hirata, Tsutomu, Ryu, Seung-Lim, Shimizu, Takashi, Yabe, Taijirou, Yamanaka, Yojiro
- Keywords
- zebrafish; dharma; bozozok; Nodal; squint; cyclops; Nieuwkoop center; Spemann organizer
- MeSH Terms
-
- Glycoproteins*
- Gene Expression Regulation, Developmental
- Nodal Signaling Ligands
- Transcription Factors*
- Blastocyst/physiology
- Body Patterning/physiology*
- Glycogen Synthase Kinase 3
- Zebrafish/embryology*
- Zebrafish/physiology
- Intercellular Signaling Peptides and Proteins*
- beta Catenin
- Intracellular Signaling Peptides and Proteins
- Calcium-Calmodulin-Dependent Protein Kinases/genetics
- Calcium-Calmodulin-Dependent Protein Kinases/metabolism
- Gastrula/physiology
- Trans-Activators*
- Animals
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism
- Proteins/genetics
- Zebrafish Proteins*
- Time Factors
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Repressor Proteins*
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism*
- Goosecoid Protein
- PubMed
- 10704853 Full text @ Mech. Dev.
Citation
Shimizu, T., Yamanaka, Y., Ryu, S., Hashimoto, H., Yabe, T., Hirata, T., Bae, Y., Hibi, M., and Hirano, T. (2000) Cooperative roles of Bozozok/Dharma and Nodal-related proteins in the formation of the dorsal organizer in zebrafish. Mechanisms of Development. 91(1-2):293-303.
Abstract
In vertebrates, specification of the dorso-ventral axis requires Wnt signaling, which leads to formation of the Nieuwkoop center and the Spemann organizer (dorsal organizer), through the nuclear accumulation of beta-catenin. Zebrafish bozozok/dharma (boz) and squint (sqt), which encode a homeodomain protein and a Nodal-related protein, respectively, are required for the formation of the dorsal organizer. The zygotic expression of boz and sqt in the dorsal blastoderm and dorsal yolk syncytial layer (YSL) was dependent on the maternally derived Wnt signal, and their expression at the late blastula and early gastrula stages was dependent on the zygotic expression of their own genes. The dorsal organizer genes, goosecoid (gsc) and chordin (din), were ectopically expressed in wild-type embryos injected with boz or sqt RNA. The expression of gsc strictly depended on both boz and sqt while the expression of din strongly depended on boz but only partially depended on sqt and cyclops (cyc, another nodal-related gene). Overexpression of boz in embryos defective in Nodal signaling elicited the ectopic expression of din but not gsc and resulted in dorsalization, implying that boz could induce part of the organizer, independent of the Nodal proteins. Furthermore, boz; sqt and boz;cyc double mutants displayed a severely ventralized phenotype with anterior truncation, compared with the single mutants, and boz;sqt;cyc triple mutant embryos exhibited an even more severe phenotype, lacking the anterior neuroectoderm and notochord, suggesting that Boz/Dharma and the Nodal-related proteins cooperatively regulate the formation of the dorsal organizer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping