PUBLICATION
Cyclic nucleotide-gated channel activation is not required for activity-dependent labeling of zebrafish olfactory receptor neurons by amino acids
- Authors
- Michel, W.C.
- ID
- ZDB-PUB-000103-6
- Date
- 1999
- Source
- Biological signals and receptors 8(6): 338-347 (Journal)
- Registered Authors
- Michel, William C.
- Keywords
- olfaction; electro-olfactogram; activity-dependent labeling; AGB; zebrafish
- MeSH Terms
-
- Agmatine/pharmacology
- Amino Acids/pharmacology*
- Animals
- Bile Acids and Salts/pharmacology
- Colforsin/pharmacology
- Cyclic AMP/metabolism
- Cyclic Nucleotide-Gated Cation Channels
- Intracellular Membranes/metabolism
- Ion Channels/physiology*
- Neurons, Afferent/drug effects
- Neurons, Afferent/physiology*
- Odorants
- Olfactory Pathways/drug effects
- Olfactory Pathways/physiology*
- Zebrafish/physiology*
- PubMed
- 10592376 Full text @ Biol. Signals Recept.
Citation
Michel, W.C. (1999) Cyclic nucleotide-gated channel activation is not required for activity-dependent labeling of zebrafish olfactory receptor neurons by amino acids. Biological signals and receptors. 8(6):338-347.
Abstract
The olfactory epithelium of fish is heterogeneous both with respect to the types of receptor cells (ORNs) present and the families of odorant receptors expressed in these cells. As a consequence of this diversity, the transduction cascade(s) activated by odorants has yet to be unambiguously established. In the current study, electrophysiological and activity-dependent labeling techniques were used to assess the role of the cyclic nucleotide-gated channel in zebrafish olfactory transduction. Both amino acid and bile salt odorants elicited robust electrophysiological responses, however, activity-dependent labeling of ORNs could be stimulated only by the amino acid odorants. An adenylate cyclase (AC) activator (forskolin) and a phosphodiesterase inhibitor (3-isobutyl-1-methylxanthine, IBMX) also elicited robust electrophysiological responses; generally larger than the responses elicited by either the amino acid or bile salt odorants. However, neither forskolin alone or a mixture of forskolin and IBMX stimulated activity-dependent labeling. Bathing the olfactory epithelium with forskolin, which presumably increased the intracellular concentration of cAMP, reduced the responses to bile salt odorants to a significantly greater extent than amino acid odorants. Collectively, these findings suggest that the transduction of amino acid input does not rely primarily on cyclic nucleotide-gated (CNG) channel activation and that CNG channel activation may be required for the transduction of bile salt input.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping