Person
Graf, Thomas
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Biography and Research Interest
*** no longer involved in zebrafish research ***
I am interested in hemopoietic stem cell biology and leukemia. In my previous work, at the EMBL in Heidelberg, we have developed a system of multipotent progenitors transformed by an avian leukemia virus which can be grown in large amounts and can be aeasily handled. Using these cells we showed a role of the transcription factors GATA-1, PU.1, C/EBP a and b in decision making towards eosinophils and myeloblasts. We are now attempting to make transgenic zebrafish in which cells of the myeloid lineage are labeled with a red fluorescent protein. The long term goal is to create bicolored fish through crossing with the GATA-1 EGFP fish from Shuo Lin, that contain green red blood cells. Embryos of these fish will then be used in time lapse experiments, as a testtube for genes that affect lineage decisions (such as the above transcription factors), as recipients for leukemia inducing genes and possibly as a tool to perform a screen for novel genes that play a role in commitment.
I am interested in hemopoietic stem cell biology and leukemia. In my previous work, at the EMBL in Heidelberg, we have developed a system of multipotent progenitors transformed by an avian leukemia virus which can be grown in large amounts and can be aeasily handled. Using these cells we showed a role of the transcription factors GATA-1, PU.1, C/EBP a and b in decision making towards eosinophils and myeloblasts. We are now attempting to make transgenic zebrafish in which cells of the myeloid lineage are labeled with a red fluorescent protein. The long term goal is to create bicolored fish through crossing with the GATA-1 EGFP fish from Shuo Lin, that contain green red blood cells. Embryos of these fish will then be used in time lapse experiments, as a testtube for genes that affect lineage decisions (such as the above transcription factors), as recipients for leukemia inducing genes and possibly as a tool to perform a screen for novel genes that play a role in commitment.
Non-Zebrafish Publications
McNagny, K.M., Pettersson, I., Rossi, F., Flamme, I., Shevchenko, A., Mann, M. and Graf, T. (1997). Thrombomucin, a Novel Cell Surface Protein that Defines Thrombocytes and Multipotent Hematopoietic Progenitors. Journal of Cell Biology, 138, 1395-1407.Sieweke, M.H., Tekotte, H. and Graf, T. (1997). Cooperative interaction of Ets-1 with USF-1 on the distal enhancer of the HIV-1 LTR. EMBO Journal, 17, 1728-1739..
Nerlov, C. and Graf, T. (1998). PU.1 induces myeloid commitment in multipotent hematopoietic progenitors. Genes & Dev. 12, 2403-2412
Nerlov, C., McNagny, K., Döderlein, G., Kowenz-Leutz, E. and Graf, T. (1998). Distinct C/EBP functions are required for eosinophil lineage commitment and maturation. Genes & Dev. 12, 2413-2423
McNagny, K.M., Sieweke, M., Döderlein, G., Graf, T. and Nerlov, C. (1998).Regulation of eosinophil-specific gene expression by a C/EBP-Ets-1 complex and GATA-1. EMBO J. 17, 3669-3680
Sieweke, M. and Graf, T. (1998). A party of transcription factors during blood cell differentiation. Curr Opin Genet Dev. 1998 8, 545-51