ZFIN ID: ZDB-PERS-990315-1
Challa, Anil Kumar
Email: akchalla@uab.edu
Address: Department of Biology University of Alabama Birmingham Birmingham, Alabama 35294
Phone: (614) 736 1361
ORCID ID: 0000-0002-3633-3304

I am broadly interested in understanding the mechanisms underlying cell movements during vertebrate embryogenesis. I have been interested in the Roundabout (Robo) family of receptor proteins and their role in a variety of developmental events. The Robo family has multiple orthologs/paralogs owing to duplication events during evolution, and to multiple isoforms possibly owing to complex molecular genetic regulation. The zebrafish provides a tractable system to study genetics and cell biology of the Robo family of proteins.

As part of a collaborative effort with Dr.Kiranam Chatti at the Institute of Life Sciences (Hyderabad, India), I got interested in protein tyrosine kinases in the zebrafish genome. One of the goals of this effort is to identify and characterize, all the tyrosine kinase genes important in specific developmental events.

I am also interested in exploiting zebrafish as a model organism in high school and undergraduate science education.

Chen, J., Baxi, K., Lipsitt, A.E., Hensch, N.R., Wang, L., Sreenivas, P., Modi, P., Zhao, X.R., Baudin, A., Robledo, D.G., Bandyopadhyay, A., Sugalski, A., Challa, A.K., Kurmashev, D., Gilbert, A.R., Tomlinson, G.E., Houghton, P., Chen, Y., Hayes, M.N., Chen, E.Y., Libich, D.S., Ignatius, M.S. (2023) Defining function of wild-type and three patient specific TP53 mutations in a zebrafish model of embryonal rhabdomyosarcoma. eLIFE. 12:
Balamurugan, K., Medishetti, R., Kotha, J., Behera, P., Chandra, K., Mavuduru, V.A., Joshi, M.B., Samineni, R., Katika, M.R., Ball, W.B., Thondamal, M., Challa, A., Chatti, K., Parsa, K.V.L. (2022) PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways. iScience. 25:103766
Lipsitt, A., Hensch, N.R., Moreno-Campos, R., Baxi, K., Chen, J., Challa, A.K., Chen, E.Y., Ignatius, M.S. (2021) Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions. Zebrafish. 18(4):293-296
Turner, A.N., Andersen, R.S., Bookout, I.E., Brashear, L.N., Davis, J.C., Gahan, D.M., Davis, J.C., Gotham, J.P., Hijaz, B.A., Kaushik, A.S., Mcgill, J.B., Miller, V.L., Moseley, Z.P., Nowell, C.L., Patel, R.K., Rodgers, M.C., Patel, R.K., Shihab, Y.A., Walker, A.P., Glover, S.R., Foster, S.D., Challa, A.K. (2018) Analysis of novel domain-specific mutations in the zebrafish ndr2/cyclops gene generated using CRISPR-Cas9 RNPs. Journal of genetics. 97:1315-1325
Foster, S.D., Glover, S.R., Turner, A.N., Chatti, K., Challa, A.K. (2018) A mixing heteroduplex mobility assay (mHMA) to genotype homozygous mutants with small indels generated by CRISPR-Cas9 nucleases. MethodsX. 6:1-5
Bhatt, J.M., Challa, A.K. (2017) First Year Course-Based Undergraduate Research Experience (CURE) Using the CRISPR/Cas9 Genome Engineering Technology in Zebrafish. Journal of microbiology & biology education. 19(1)
Challa, A.K., and Chatti, K. (2013) Conservation and Early Expression of Zebrafish Tyrosine Kinases Support the Utility of Zebrafish as a Model for Tyrosine Kinase Biology. Zebrafish. 10(3):264-74
Rana, N., Moond, M., Marthi, A., Bapatla, S., Sarvepalli, T., Chatti, K., and Challa, A.K. (2010) Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action: an effective system for experiments in chemical biology. Zebrafish. 7(1):69-81
Challa, A.K., McWhorter, M.L., Wang, C., Seeger, M.A., and Beattie, C.E. (2005) Robo3 isoforms have distinct roles during zebrafish development. Mechanisms of Development. 122(10):1073-1086
Challa, A. K. (2003) Direct Submission of Mapping Data (Anil Kumar Challa). Direct Submission of Mapping Data.
Challa, A.K. (2003) Identification and functional analysis of Zebrafish orthologs of genes. Ph.D. Thesis.
Challa, A.K., Beattie, C.E., and Seeger, M.A. (2001) Identification and characterization of roundabout orthologs in zebrafish. Mechanisms of Development. 101:243-247

Google Scholar

1: Lewis WR, Malarkey EB, Tritschler D, Bower R, Pasek RC, Porath JD, Birket SE, Saunier S, Antignac C, Knowles MR, Leigh MW, Zariwala MA, Challa AK, Kesterson RA, Rowe SM, Drummond IA, Parant JM, Hildebrandt F, Porter ME, Yoder BK, Berbari NF. Mutation of Growth Arrest Specific 8 Reveals a Role in Motile Cilia Function and Human Disease. PLoS Genet. 2016 Jul 29;12(7):e1006220. doi:10.1371/journal.pgen.1006220. eCollection 2016 Jul. PubMed PMID: 27472056.

2: Challa, Anil K., Evan R. Boitet, Ashley N. Turner, Larry W. Johnson, Daniel Kennedy, Ethan R. Downs, Katherine M. Hymel, Alecia K. Gross, and Robert A. Kesterson. (2016) "Novel Hypomorphic Alleles of the Mouse Tyrosinase Gene Induced by CRISPR-Cas9 Nucleases Cause Non-Albino Pigmentation Phenotypes." PLoS ONE 11, no. 5: e0155812.

3: Chen HY, Challa AK, Varki A. (2006) 9-O-acetylation of exogenously added ganglioside GD3. The GD3 molecule induces its own O-acetylation machinery. J Biol Chem. 281(12):7825-33.