Person
Wang, Vivien Ya-Fan
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Biography and Research Interest
Our lab has been focused on understanding the signal transduction mediated by the nuclear factor kappaB (NF-kB) and IkB proteins. The mammalian Rel/NF-kBfamily of transcription factors plays a critical role in diverse physiological processes including the immune response, inflammation, cell proliferation and survival. A wide variety of signals activate NF-kB driven gene expression through different NF-kB signaling pathways in a stimulus- and cell type-dependent manner. Due to the diverse regulatory mechanisms acting on NF-kB, the signal dependent activation of NF-kB remains a challenging area of research. Using the knowledge and experience in the area of biochemistry, structural biology, cellular and molecular biology, I have been committed to study the molecular mechanism of optimal regulation on gene expression through the NF-kB signaling which is extremely important in the field of immunology and cancer biology.
Non-Zebrafish Publications
1. Xiao, LF; Li, Y; Lian, H; Liu, X; Wen, Y; Chen, X; Huang, W; Li, B; Luo, L; Wang, X; Tutt, C; Zheng, J#; Wang, VYF#; Shao, NY#. Comprehensive metagenomic analysis of the giant panda’s oral microbiome reveals distinct taxonomic and functional characteristics. Anim Microbiome. 2025 Oct 19; 7(1):114. DOI: 10.1186/s42523-025-00475-z.
2. Zhang, Y; Ma X, Zhu, M; Wang, VYF#; Guo, J#. Progress and Prospects in FRET for the Investigation of Protein-Protein Interactions. Biosensors. 2025 Sep 19; 15(9):624. DOI: 10.3390/bios15090624.
3. Morgan, D; Zhang, B; Fidan, K; Pan, W; Vaiyapuri, TS; Raju, A; Hei, D; See, TY; Sari, IN; Chan, JW; Majee, P; Balachander, A; Yu, J; Wong, AH; Mok, MMH; Foo, SH; Tang, WL; Ang, N; Tan, I; Peng, YF; Jaynes, P; Xu, S; Ghosh, G; Shahabi, S; Jeyasekharan, AD; Ikawa, M; Zhang, Y; Howland, SW; Lau, MC; Wang, VYF; Lam, KP; Tergaonkar, V. The transcription complex p52-ETS1 is essential for germinal center formation. Nat Immunol. 2025 Jul 25. DOI: 10.1038/s41590-025-02236-1.
4. Liang, Y; Xiong, XY; Lin, GW; Bai, X; Li, F; Ko, JM; Zhou, YH; Xu, AY; Liu, SQ; He, S; Wei, PP; Chen, QY; Tang, LQ; Wang, VYF; Mai, HQ; Luo, CL; Zeng, Y; Lung, ML; Ji, M; Bei, JX. Integrative Transcriptome-Wide Association Study With Expression Quantitative Trait Loci Colocalization Identifies a Causal VAMP8 Variant for Nasopharyngeal Carcinoma Susceptibility. Adv Sci. 2025 Mar;12(11):e2412580. DOI: 10.1002/advs.202412580.
5. Zhou, YQ; Jiang, JX; He, S; Li, YQ; Cheng, XX; Liu, SQ; Wei, PP; Guan, XY; Ong, CK Wang, VYF; Luo, CL; Bei, JX. Epstein-Barr virus hijacks histone demethylase machinery to drive epithelial malignancy progression through KDM5B upregulation. Signal Transduct Target Ther. 2025 Mar 10;10(1):83. DOI: 10.1038/s41392-025-02163-5.
6. Li, T; Shahabi, S; Biswas, T; Tsodikov, O; Pan, W; Huang, D; Wang, VYF; Wang, Y; Ghosh, G. Transient interactions modulate affinity of NF-κB transcription factors for DNA. PNAS. 2024 Jun 4;121(23):e2405555121. DOI: 10.1073/pnas.2405555121.
7. Pan, W; Meshcheryakov, VA; Li, T; Wang, Y; Ghosh, G; Wang, VYF#. Structures of NF-κB p52 homodimer-DNA complexes rationalize binding mechanisms and transcription activation. eLife. 2023, Feb 13; 12:e86258. DOI: https://doi.org/10.7554/eLife.86258.
8. Wang, P; Wu, R; Jia, Y; Tang, P; Wei, B; Zhang, Q; Wang, VYF; Ru, Y. Inhibition and structure-activity relationship of dietary flavone against three Loop 1-type human gut microbial β-glucuronidases. Int J Biol Macromol. 2022, Sep 9; S0141-8130(22)01940-7.
9. Pan, W; Deng, L; Wang, H; Wang, VYF#. Atypical IκB Bcl3 enhances the generation of the NF-κB p52 homodimer. Front Cell Dev Biol. 2022, Aug 5; 10:930619.
10. Tang, L; Zhang D; Han, P; Kang, X; Zheng, A; Xu Z; Zhao X; Wang, VYF; Qi, J; Wang, Q; Liu, K; Gao, GF. Structural basis of SARS-CoV-2 and its variants binding to intermediate horseshoe bar ACE2. Int J Biol Sci. 2022, Jul 11; 18(12):4658-4668.
11. Ghosh, G; Wang, VYF#. Origin of the functional distinctiveness of NF-κB/p52. Front Cell Dev Biol. 2021, 9:764164. DOI: https://doi.org/10.3389/fcell.2021.764164.
12. Lin, GW; Xu, CG; Chen, K; Huang, HQ; Chen, JP; Song, B; Chan, JKC; Li, WY; Liu, WP; Shih, LY; Chuang, WY; Kim, WS; Tan, W; Peng, RJ; Laurensia, Y; Cheah, DMZ; Huang, DC; Cheng, CL; Su, Y-J; Tan, SY; Ng, SB; Tang, TPL; Han, KD; Wang, VYF; Jia, WH; Pei, Z; Li, YJ; Gao, S; Shi, YY; Hu, ZB; Zhang, F; Zhang, B; Zeng, YX; Shen, HB; He, L; Ong, CK; Lim, ST; Chanock, S; Kwong, YL; Lin, D; Rothman, N; Khor, CC; Lan, Q; and Bei, JX. Genetic Risk of Extranodal Natural Killer T-Cell Lymphoma: A Genome-Wide Association Study in Multiple Populations. Lancet Oncol. 2020, 21 (2), 306-316. DOI: https://doi.org/10.1016/S1470-2045(19)30799-5
13. Mulero, MC*; Wang, VYF*; Huxford, T; Ghosh, G. Genome reading by the NF-κB transcription factors. Nucleic Acids Res. 2019, 47(19), 9967-9989. DOI: https://doi.org/10.1093/nar/gkz739
14. Kwasnik, A*; Wang, VYF*; Krzyszton, M; Gozdek, A; Zakrzewska-Placzek, M; Stepniak, K; Poznanski, J; Tong, L; and Kufel, J. Arabidopsis DXO1 links RNA turnover and chloroplast function independently of its enzymatic activity. Nucleic Acids Res. 2019, 47(9), 4751-4764. http://doi.org/10.1093/nar/gkz100
15. Meng, Y; Ren, Z; Xu, F; Zhou, X; Song, C; Wang, VYF; Liu, W; Lu, L; Thomson, JA; Chen, G. Nicotinamide Promotes Cell Survival and Differentiation as Kinase Inhibitor in Human Pluripotent Stem Cells. Stem Cell Reports. 2018, 11(6):1347-1356.
16. Mulero, MC; Shahabi, S; Ko, MS; Schiffer, JM; Huang, D-B; Wang, VYF; Amaro, RE; Huxford, T; Ghosh, G. Protein Cofactors Are Essential for High-Affinity DNA Binding by the Nuclear Factor κB RelA Subunit. Biochemistry. 2018, 57(20):2943-2957.
17. Mulero, MC; Huang, D-B; Nguyen, HT; Wang, VYF; Li, Y; Biswas, T; Ghosh, G. DNA-binding affinity and transcriptional activity of the RelA homodimer of nuclear factor κB are not correlated. J Biol Chem. 2017, 292(46):18821-18830. DOI:10.1074/jbc.M117.813980.
18. Wang, VYF; Li Y; Kim, D; Zhong, XY; Ghassemian, M; and Ghosh, G. Bcl3 Phosphorylation by Akt, Erk2 and IKK1 is Required for Its Activation. Mol Cell. 2017, 67(1), 1-14. DOI: https://doi.org/10.1016/j.molcel.2017.06.011.
19. Fusco, AJ; Mazumder, A; Wang, VYF; Tao, Z; Ware, C; and Ghosh, G. The NF-κB subunit RelB controls p100 processing by competing with the kinases NIK and IKK1 for binding to p100. Sci Signal. 2016, 9(447):ra96. DOI: 10.1126/scisignal.aad9413.
20. Sansó, M; Levin, RS; Lipp JJ; Wang, VYF; Greifenberg, AK; Quezada, EM; Ali, A; Ghosh, A; Larochelle, S; Rana, TM; Geyer, M; Tong, L; Shokat, KM; Fisher, RP. P-TEFb regulation of transcription termination factor Xrn2 revealed by a chemical genetic screen for Cdk9 substrates. Genes Dev. 2016, 30(1), 117-131.
21. Wang, VYF; Jiao, X; Kiledjian, M; and Tong, L. Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes. Nucleic Acids Res. 2015, 43(13), 6596-6606.
22. Wang, VYF; Huang, W; Asagiri, M; Spann, N; Hoffmann, A; Glass, C; and Ghosh, G. The transcriptional specificity of NF-κB Dimers is coded within the κB DNA Response elements. Cell Rep. 2012, 2(4), 824-839.
23. Ghosh, G; Wang, VYF; Huang, D-B; and Fusco, AJ. NF-κB Regulation: Lessons from Structures. Immunological Rev. 2012, 246, 36-58.
24. Fusco, AJ; Huang, D-B; Miller, D; Wang, VYF; Vu, D; and Ghosh, G. NF-kappaB p52:RelB heterodimer recognizes two classes of kappaB sites with two distinct modes. EMBO Rep. 2009, 10, 152-159.
25. Moorthy, AK; Huang, D-B; Wang, VYF; Vu, D; and Ghosh, G. X-ray structure of a NFkappaB p50/RelB/DNA complex reveals assembly of multiple dimers on tandem kappaB sites. J Mol Biol. 2007, 373, 723-734.
26. Moorthy, AK; Savinova, OV; Ho, JQ; Wang, VYF; Vu, D; and Ghosh, G. The 20S proteasome processes NF-kappaB1 p105 into p50 in a translation-independent manner. EMBO J. 2006, 25, 1945-1956.