ZFIN ID: ZDB-PERS-000424-1
BIOGRAPHY AND RESEARCH INTERESTS
Brian Perkins Lab
||Associate Staff, Department of Ophthalmic Research, Cole Eye Institute
Associate Professor of Ophthalmology
9500 Euclid Ave. Cleveland, OH 44195
Our lab studies the development of the vertebrate retina. The retina of a zebrafish is very similar to that of humans in both structure and function, so the genes required to make a zebrafish retina should be the same ones needed to make a human retina. To better understand the processes involved, we look for mutant zebrafish that have abnormal retinas. To facilitate this, transgenic zebrafish that express GFP in the rod photoreceptors have been integrated into the genetic screens for mutants. We are also looking at genes that control ciliogenesis in the retina and result in photoreceptor cell death. Future work on these mutants will help identify genes required for normal retinal development and will likely have relevance to human blindness disorders such as macular degeneration and retinitis pigmentosa.
Lessieur, E.M., Song, P., Nivar, G.C., Piccillo, E.M., Fogerty, J., Rozic, R., Perkins, B.D. (2019) Ciliary genes arl13b, ahi1 and cc2d2a differentially modify expression of visual acuity phenotypes but do not enhance retinal degeneration due to mutation of cep290 in zebrafish. PLoS One. 14:e0213960
Zhu, L., Chen, L., Yan, L., Perkins, B.D., Li, S., Li, B., Xu, H.A., Li, X.J. (2019) Mutant Ahi1 Affects Retinal Axon Projection in Zebrafish via Toxic Gain of Function. Frontiers in Cellular Neuroscience. 13:81
Miller, A.H., Howe, H.B., Krause, B.M., Friedle, S.A., Banks, M.I., Perkins, B.D., Wolman, M.A. (2018) Pregnancy associated plasma protein-aa (Pappaa) regulates photoreceptor synaptic development to mediate visually guided behavior. The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(22):5220-5236
Radhakrishnan, U., Alsrhani, A., Sundaramoorthi, H., Khandekar, G., Kashyap, M., Fuchs, J.L., Perkins, B.D., Omori, Y., Jagadeeswaran, P. (2017) Intraflagellar transport proteins are involved in thrombocyte filopodia formation and secretion. Platelets. 29(8):811-820
Bell, B.A., Yuan, A., Dicicco, R.M., Fogerty, J., Lessieur, E.M., Perkins, B.D. (2016) The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography. Experimental Eye Research. 153:65-78
Daniele, L.L., Emran, F., Lobo, G.P., Gaivin, R.J., Perkins, B.D. (2016) Mutation of wrb, a Component of the Guided Entry of Tail-Anchored Protein Pathway, Disrupts Photoreceptor Synapse Structure and Function. Investigative ophthalmology & visual science. 57:2942-54
DiCicco, R.M., Bell, B.A., Kaul, C., Hollyfield, J.G., Anand-Apte, B., Perkins, B.D., Tao, Y.K., Yuan, A. (2014) Retinal Regeneration Following OCT-guided Laser Injury in Zebrafish. Investigative ophthalmology & visual science. 55(10):6281-8
Wang, H., Chen, X., Dudinsky, L., Patenia, C., Chen, Y., Li, Y., Wei, Y., Abboud, E.B., Al-Rajhi, A.A., Lewis, R.A., Lupski, J.R., Mardon, G., Gibbs, R.A., Perkins, B.D., and Chen, R. (2011) Exome capture sequencing identifies a novel mutation in BBS4. Molecular Vision. 17:3529-3540
Hudak, L.M., Lunt, S., Chang, C.H., Winkler, E., Flammer, H., Lindsey, M., and Perkins, B. (2010) The Intraflagellar Transport Protein Ift80 is essential for Photoreceptor Survival in a Zebrafish Model of Jeune Asphyxiating Thoracic Dystrophy. Investigative ophthalmology & visual science. 51(7):3792-3799
Insinna, C., Pathak, N., Perkins, B., Drummond, I., and Besharse, J.C. (2008) The homodimeric kinesin, Kif17, is essential for vertebrate photoreceptor sensory outer segment development. Developmental Biology. 316(1):160-170
Gross, J.M., Perkins, B.D., Amsterdam, A., Egana, A., Darland, T., Matsui, J.I., Sciascia, S., Hopkins, N., and Dowling, J.E. (2005) Identification of Zebrafish Insertional Mutants with Defects in Visual System Development and Function. Genetics. 170(1):245-261
*Intody, Z., *Perkins, B.D., Wilson, J.H., and Wensel, T.G. (2000) Blocking transcription of the human rhodopsin gene by triplex-mediated DNA photocrosslinking. Nucleic Acids Research. 28(21):4283-4290.
Sargent, R.G., Meservy, J.L., Perkins, B.D., Kilburn, A.E., Intody, Z., Adair, G.M., Nairn, R.S., and Wilson, J.H. (2000) Role of the nucleotide excision repair gene ERCC1 in formation of recombination-dependent rearrangements in mammalian cells. Nucleic Acids Research. 28(19):3771-3778.
Perkins, B.D., Wensel, T.G., Vasquez, K.M., and Wilson, J.H. (1999) Psoralen photocrosslinking by triplex-forming oligonucleotides at multiple site sin the human rhodopsin gene. Biochemistry. 38(39):12850-12859.
Perkins, B.D., Wilson, J.H., Wensel, T.G., and Vasquez, K.M. (1998) Triplex targets in the human rhodopsin gene. Biochemistry. 37(32):11315-11322