ZFIN ID: ZDB-PERS-000424-1
Perkins, Brian
Email: perkinb2@ccf.org
Affiliation: Brian Perkins Lab
Address: Associate Staff, Department of Ophthalmic Research, Cole Eye Institute Associate Professor of Ophthalmology Cleveland Clinic 9500 Euclid Ave. Cleveland, OH 44195
Country: United States
Phone: (216) 444-9683

Our lab studies the development of the vertebrate retina. The retina of a zebrafish is very similar to that of humans in both structure and function, so the genes required to make a zebrafish retina should be the same ones needed to make a human retina. To better understand the processes involved, we look for mutant zebrafish that have abnormal retinas. To facilitate this, transgenic zebrafish that express GFP in the rod photoreceptors have been integrated into the genetic screens for mutants.  We are also looking at genes that control ciliogenesis in the retina and result in photoreceptor cell death.  Future work on these mutants will help identify genes required for normal retinal development and will likely have relevance to human blindness disorders such as macular degeneration and retinitis pigmentosa.

Grabinski, S.E., Parsana, D., Perkins, B.D. (2023) Comparative analysis of transcriptional changes in zebrafish cep290 and bbs2 mutants by RNA-seq reveals upregulation of inflammatory and stress-related pathways. Frontiers in molecular neuroscience. 16:11488401148840
Fogerty, J., Song, P., Boyd, P., Grabinski, S.E., Hoang, T., Reich, A., Cianciolo, L.T., Blackshaw, S., Mumm, J.S., Hyde, D.R., Perkins, B.D. (2022) Notch inhibition promotes regeneration and immunosuppression supports cone survival in a zebrafish model of inherited retinal dystrophy. The Journal of neuroscience : the official journal of the Society for Neuroscience. 42(26):5144-5158
Banerjee, S., Ranspach, L.E., Luo, X., Cianciolo, L.T., Fogerty, J., Perkins, B.D., Thummel, R. (2022) Vision and sensorimotor defects associated with loss of Vps11 function in a zebrafish model of genetic leukoencephalopathy. Scientific Reports. 12:3511
Song, P., Fogerty, J., Cianciolo, L.T., Stupay, R., Perkins, B.D. (2020) Cone Photoreceptor Degeneration and Neuroinflammation in the Zebrafish Bardet-Biedl Syndrome 2 (bbs2) Mutant Does Not Lead to Retinal Regeneration. Frontiers in cell and developmental biology. 8:578528
Pollock, L.M., Perkins, B., Anand-Apte, B. (2020) Primary cilia are present on endothelial cells of the hyaloid vasculature but are not required for the development of the blood-retinal barrier. PLoS One. 15:e0225351
Solanki, A.K., Kondkar, A.A., Fogerty, J., Su, Y., Kim, S.H., Lipschutz, J.H., Nihalani, D., Perkins, B.D., Lobo, G.P. (2020) A Functional Binding Domain in the Rbpr2 Receptor Is Required for Vitamin A Transport, Ocular Retinoid Homeostasis, and Photoreceptor Cell Survival in Zebrafish. Cells. 9(5):
Fogerty, J., Stepanyan, R., Cianciolo, L.T., Tooke, B.P., Perkins, B.D. (2019) Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance. Scientific Reports. 9:10302
Lessieur, E.M., Song, P., Nivar, G.C., Piccillo, E.M., Fogerty, J., Rozic, R., Perkins, B.D. (2019) Ciliary genes arl13b, ahi1 and cc2d2a differentially modify expression of visual acuity phenotypes but do not enhance retinal degeneration due to mutation of cep290 in zebrafish. PLoS One. 14:e0213960
Zhu, L., Chen, L., Yan, L., Perkins, B.D., Li, S., Li, B., Xu, H.A., Li, X.J. (2019) Mutant Ahi1 Affects Retinal Axon Projection in Zebrafish via Toxic Gain of Function. Frontiers in Cellular Neuroscience. 13:81
Song, P., Perkins, B.D. (2018) Developmental expression of the zebrafish Arf-like small GTPase paralogs arl13a and arl13b. Gene expression patterns : GEP. 29:82-87
Miller, A.H., Howe, H.B., Krause, B.M., Friedle, S.A., Banks, M.I., Perkins, B.D., Wolman, M.A. (2018) Pregnancy associated plasma protein-aa (Pappaa) regulates photoreceptor synaptic development to mediate visually guided behavior. The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(22):5220-5236
Radhakrishnan, U., Alsrhani, A., Sundaramoorthi, H., Khandekar, G., Kashyap, M., Fuchs, J.L., Perkins, B.D., Omori, Y., Jagadeeswaran, P. (2017) Intraflagellar transport proteins are involved in thrombocyte filopodia formation and secretion. Platelets. 29(8):811-820
Lessieur, E.M., Fogerty, J., Gaivin, R.J., Song, P., Perkins, B.D. (2017) The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science. 58:448-460
Bell, B.A., Yuan, A., Dicicco, R.M., Fogerty, J., Lessieur, E.M., Perkins, B.D. (2016) The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography. Experimental Eye Research. 153:65-78
Song, P., Dudinsky, L., Fogerty, J., Gaivin, R., Perkins, B.D. (2016) Arl13b Interacts With Vangl2 to Regulate Cilia and Photoreceptor Outer Segment Length in Zebrafish. Investigative ophthalmology & visual science. 57:4517-4526
Daniele, L.L., Emran, F., Lobo, G.P., Gaivin, R.J., Perkins, B.D. (2016) Mutation of wrb, a Component of the Guided Entry of Tail-Anchored Protein Pathway, Disrupts Photoreceptor Synapse Structure and Function. Investigative ophthalmology & visual science. 57:2942-54
Fogerty, J., Denton, K., Perkins, B.D. (2016) Mutations in the Dynein1 Complex are Permissible for Basal Body Migration in Photoreceptors but Alter Rab6 Localization. Advances in experimental medicine and biology. 854:209-15
Babino, D., Perkins, B.D., Kindermann, A., Oberhauser, V., von Lintig, J. (2015) The role of 11-cis-retinyl esters in vertebrate cone vision. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(1):216-26
DiCicco, R.M., Bell, B.A., Kaul, C., Hollyfield, J.G., Anand-Apte, B., Perkins, B.D., Tao, Y.K., Yuan, A. (2014) Retinal Regeneration Following OCT-guided Laser Injury in Zebrafish. Investigative ophthalmology & visual science. 55(10):6281-8
Krock, B.L., Perkins, B.D. (2014) The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors. PLoS One. 9:e104661
Wasfy, M.M., Matsui, J.I., Miller, J., Dowling, J.E., Perkins, B.D. (2014) myosin 7aa(-/-) mutant zebrafish show mild photoreceptor degeneration and reduced electroretinographic responses. Experimental Eye Research. 122:65-76
Ramsey, M., and Perkins, B.D. (2013) Basal bodies exhibit polarized positioning in zebrafish cone photoreceptors. The Journal of comparative neurology. 521(8):1803-1816
Wang, H., Chen, X., Dudinsky, L., Patenia, C., Chen, Y., Li, Y., Wei, Y., Abboud, E.B., Al-Rajhi, A.A., Lewis, R.A., Lupski, J.R., Mardon, G., Gibbs, R.A., Perkins, B.D., and Chen, R. (2011) Exome capture sequencing identifies a novel mutation in BBS4. Molecular Vision. 17:3529-3540
Hudak, L.M., Lunt, S., Chang, C.H., Winkler, E., Flammer, H., Lindsey, M., and Perkins, B. (2010) The Intraflagellar Transport Protein Ift80 is essential for Photoreceptor Survival in a Zebrafish Model of Jeune Asphyxiating Thoracic Dystrophy. Investigative ophthalmology & visual science. 51(7):3792-3799
Perkins, B.D., and Fadool, J.M. (2010) Chapter 7 - Photoreceptor Structure and Development Analyses using GFP Transgenes. Methods in cell biology. 100:205-218
Krock, B.L., Mills-Henry, I., and Perkins, B. (2009) Retrograde Intraflagellar Transport by Cytoplasmic Dynein-2 is Required for Outer Segment Extension in Vertebrate Photoreceptors but not Arrestin Translocation. Investigative ophthalmology & visual science. 50(11):5463-5471
Lunt, S.C., Haynes, T., and Perkins, B.D. (2009) Zebrafish ift57, ift88, and ift172 intraflagellar transport mutants disrupt cilia but do not affect hedgehog signaling. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(7):1744-1759
Sukumaran, S., and Perkins, B.D. (2009) Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants. Vision Research. 49(4):479-489
Krock, B.L., and Perkins, B.D. (2008) The intraflagellar transport protein IFT57 is required for cilia maintenance and regulates IFT-particle-kinesin-II dissociation in vertebrate photoreceptors. Journal of Cell Science. 121(Pt 11):1907-1915
Insinna, C., Pathak, N., Perkins, B., Drummond, I., and Besharse, J.C. (2008) The homodimeric kinesin, Kif17, is essential for vertebrate photoreceptor sensory outer segment development. Developmental Biology. 316(1):160-170
Gross, J.M., and Perkins, B.D. (2008) Zebrafish mutants as models for congenital ocular disorders in humans. Molecular reproduction and development. 75(3):547-555
Krock, B.L., Bilotta, J., and Perkins, B.D. (2007) Noncell-autonomous photoreceptor degeneration in a zebrafish model of choroideremia. Proceedings of the National Academy of Sciences of the United States of America. 104(11):4600-4605
Perkins, B.D., Nicholas, C.S., Baye, L.M., Link, B.A., and Dowling, J.E. (2005) dazed gene is necessary for late cell type development and retinal cell maintenance in the zebrafish retina. Developmental Dynamics : an official publication of the American Association of Anatomists. 233(2):680-694
Gross, J.M., Perkins, B.D., Amsterdam, A., Egana, A., Darland, T., Matsui, J.I., Sciascia, S., Hopkins, N., and Dowling, J.E. (2005) Identification of Zebrafish Insertional Mutants with Defects in Visual System Development and Function. Genetics. 170(1):245-261
Perkins, B.D., Fadool, J.M., and Dowling, J.E. (2004) Photoreceptor structure and development: analyses using GFP transgenes. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:315-331
Perkins, B.D., Kainz, P.M., O'Malley, D.M., and Dowling, J.E. (2002) Transgenic expression of a GFP-rhodopsin COOH-terminal fusion protein in zebrafish rod photoreceptors. Visual neuroscience. 19(3):257-264
Perkins, B.D. and Dowling, J.E. (2001) Photoreceptor-specific expression of GFP in the zebrafish, Danio rerio. Investigative ophthalmology & visual science. 42(4):1953

*Intody, Z., *Perkins, B.D., Wilson, J.H., and Wensel, T.G. (2000) Blocking transcription of the human rhodopsin gene by triplex-mediated DNA photocrosslinking. Nucleic Acids Research. 28(21):4283-4290.
*Co-first authors

Sargent, R.G., Meservy, J.L., Perkins, B.D., Kilburn, A.E., Intody, Z., Adair, G.M., Nairn, R.S., and Wilson, J.H. (2000) Role of the nucleotide excision repair gene ERCC1 in formation of recombination-dependent rearrangements in mammalian cells. Nucleic Acids Research. 28(19):3771-3778.

Perkins, B.D., Wensel, T.G., Vasquez, K.M., and Wilson, J.H. (1999) Psoralen photocrosslinking by triplex-forming oligonucleotides at multiple site sin the human rhodopsin gene. Biochemistry. 38(39):12850-12859.

Perkins, B.D., Wilson, J.H., Wensel, T.G., and Vasquez, K.M. (1998) Triplex targets in the human rhodopsin gene. Biochemistry. 37(32):11315-11322