Morpholino

MO1-wnt9a

ID

ZDB-MRPHLNO-110321-2

Name

MO1-wnt9a

Previous Names

wnt9a MO (1)

Target

wnt9a (1)

Sequence

5' - CCAGGAGAAGGTGTCCATCCAGCAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Translation-blocking MO.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO1-wnt9a

Expressed Gene	Anatomy	Figures
col2a1a	trabecula cranii	Fig. 7 from Curtin et al., 2011
dlx2a	migratory neural crest neural crest pharyngeal arch 1 cranial neural crest cell pharyngeal arch 2 cranial neural crest cell whole organism	Fig. 6 from Kamel et al., 2013 Fig. 6 from Curtin et al., 2011
edn1	migratory neural crest neural crest whole organism	Fig. 6 from Kamel et al., 2013
ednraa	migratory neural crest neural crest whole organism	Fig. 6 from Kamel et al., 2013
fgf3	hypothalamus postero-ventral region midbrain hindbrain boundary optic stalk pharyngeal pouch	Fig. 1 from Wu et al., 2015
foxd3	neural crest	Fig. 6 from Kamel et al., 2013
gsc	pharyngeal arch 1 cranial neural crest cell pharyngeal arch 2 cranial neural crest cell	Fig. 6 from Curtin et al., 2011
myb	ventral aorta	Fig. 2, Fig. 4 from Grainger et al., 2019
nkx3-2	pharyngeal arch 1 mesenchyme whole organism	Fig. 6 from Kamel et al., 2013
shha	oral epithelium stomodeum	Fig. 7 from Curtin et al., 2011
sox9a	neural crest pharyngeal arch 1 pharyngeal arch 2 (not) pharyngeal arch 3-7	Fig. 6 from Kamel et al., 2013 Fig. 7 from Curtin et al., 2011
sox10	cranial neural crest cell neural crest otic vesicle	Fig. 6 from Kamel et al., 2013 Fig. 6 from Curtin et al., 2011

Phenotype

Phenotype resulting from MO1-wnt9a

Phenotype	Fish	Figures
cranial neural crest cell lateralized, abnormal	TU + MO1-wnt9a	Fig. 7 from Curtin et al., 2011
cranial neural crest cell dorsal region mislocalised laterally, abnormal	zf220Tg + MO1-wnt9a (TU)	Fig. 5 from Curtin et al., 2011
embryonic neurocranium morphogenesis decreased process quality, abnormal	nu12Tg + MO1-wnt9a	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage aplastic, abnormal	TU + MO1-wnt9a	Fig. 3 , Fig. 5 , Fig. 7 from Curtin et al., 2011
ethmoid cartilage decreased length, abnormal	vu234Tg + MO1-wnt9a	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage has fewer parts of type chondrocyte, abnormal	nu12Tg + MO1-wnt9a	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage chondrocyte disorganized, abnormal	nu12Tg + MO1-wnt9a	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage chondrocyte shape, abnormal	nu12Tg + MO1-wnt9a	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage chondrocyte proliferation decreased occurrence, abnormal	vu234Tg + MO1-wnt9a	Fig. 2 from Dougherty et al., 2013
head hypoplastic, abnormal	TU + MO1-wnt9a	Fig. 3 from Curtin et al., 2011
neural crest cell development disrupted, abnormal	zf220Tg + MO1-wnt9a (TU)	Fig. 5 , Fig. 7 from Curtin et al., 2011
neural crest cell migration disrupted, abnormal	zf220Tg + MO1-wnt9a (TU)	Fig. 5 from Curtin et al., 2011
neurocranial trabecula chondrocyte circular, abnormal	TU + MO1-wnt9a	Fig. 3 from Curtin et al., 2011
neurocranial trabecula chondrocyte disorganized, abnormal	TU + MO1-wnt9a	Fig. 3 from Curtin et al., 2011
neurocranial trabecula proximal region decreased length, abnormal	TU + MO1-wnt9a	Fig. 3 , Fig. 5 from Curtin et al., 2011
neurocranial trabecula proximal region truncated, abnormal	TU + MO1-wnt9a	Fig. 7 from Curtin et al., 2011
parachordal cartilage postero-lateral region aplastic, abnormal	TU + MO1-wnt9a	Fig. 3 from Curtin et al., 2011
pharyngeal arch 1 mesenchyme nkx3-2 expression decreased amount, abnormal	WT + MO1-wnt9a	Fig. 6 from Kamel et al., 2013
pharyngeal arch 3-7 morphology, abnormal	TU + MO1-wnt9a	Fig. 7 from Curtin et al., 2011
stomodeum shape, abnormal	TU + MO1-wnt9a	Fig. 7 from Curtin et al., 2011
trabecula communis aplastic, abnormal	zf220Tg + MO1-wnt9a (TU)	Fig. 5 from Curtin et al., 2011
ventral mandibular arch aplastic, abnormal	TU + MO1-wnt9a	Fig. 3 from Curtin et al., 2011
whole organism nkx3-2 expression decreased amount, abnormal	WT + MO1-wnt9a	Fig. 6 from Kamel et al., 2013

Phenotype of all Fish created by or utilizing MO1-wnt9a

Phenotype	Fish	Conditions	Figures
neural crest cell development disrupted, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 7 from Curtin et al., 2011
head hypoplastic, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 3 from Curtin et al., 2011
pharyngeal arch 3-7 morphology, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 7 from Curtin et al., 2011
neurocranial trabecula proximal region truncated, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 7 from Curtin et al., 2011
parachordal cartilage postero-lateral region aplastic, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 3 from Curtin et al., 2011
ethmoid cartilage aplastic, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 3 , Fig. 7 from Curtin et al., 2011
ventral mandibular arch aplastic, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 3 from Curtin et al., 2011
stomodeum shape, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 7 from Curtin et al., 2011
neurocranial trabecula proximal region decreased length, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 3 from Curtin et al., 2011
neurocranial trabecula chondrocyte disorganized, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 3 from Curtin et al., 2011
cranial neural crest cell lateralized, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 7 from Curtin et al., 2011
neurocranial trabecula chondrocyte circular, abnormal	TU + MO1-wnt9a	standard conditions	Fig. 3 from Curtin et al., 2011
whole organism nkx3-2 expression decreased amount, abnormal	WT + MO1-wnt9a	standard conditions	Fig. 6 from Kamel et al., 2013
pharyngeal arch 1 mesenchyme nkx3-2 expression decreased amount, abnormal	WT + MO1-wnt9a	standard conditions	Fig. 6 from Kamel et al., 2013
embryonic neurocranium morphogenesis decreased process quality, abnormal	nu12Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage chondrocyte shape, abnormal	nu12Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage has fewer parts of type chondrocyte, abnormal	nu12Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage chondrocyte disorganized, abnormal	nu12Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage decreased length, abnormal	nu12Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage decreased length, abnormal	vu234Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
embryonic neurocranium morphogenesis decreased process quality, abnormal	vu234Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage chondrocyte proliferation decreased occurrence, abnormal	vu234Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ethmoid cartilage aplastic, abnormal	zf220Tg + MO1-wnt9a (TU)	standard conditions	Fig. 5 from Curtin et al., 2011
trabecula communis aplastic, abnormal	zf220Tg + MO1-wnt9a (TU)	standard conditions	Fig. 5 from Curtin et al., 2011
cranial neural crest cell dorsal region mislocalised laterally, abnormal	zf220Tg + MO1-wnt9a (TU)	standard conditions	Fig. 5 from Curtin et al., 2011
neurocranial trabecula proximal region decreased length, abnormal	zf220Tg + MO1-wnt9a (TU)	standard conditions	Fig. 5 from Curtin et al., 2011
neural crest cell migration disrupted, abnormal	zf220Tg + MO1-wnt9a (TU)	standard conditions	Fig. 5 from Curtin et al., 2011
neural crest cell development disrupted, abnormal	zf220Tg + MO1-wnt9a (TU)	standard conditions	Fig. 5 from Curtin et al., 2011
ethmoid cartilage decreased length, abnormal	zf393Tg + MO1-wnt9a	standard conditions	Fig. 2 from Dougherty et al., 2013
ventral aorta myb expression decreased amount, abnormal	WT + MO1-egfra + MO1-wnt9a	standard conditions	Fig. 4 from Grainger et al., 2019
ventral aorta myb expression decreased amount, abnormal	WT + MO1-wnt9a + MO2-fzd9b	standard conditions	Fig. 2 from Grainger et al., 2019
ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal	y171Tg; zf169Tg + MO1-wnt9a	standard conditions	Fig. 3 from Grainger et al., 2016

Citations