ZFIN Morpholino: MO2-cftr

Target Location

Genome Build: GRCz11Chromosome: 18

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO2-cftr

Expressed Gene	Anatomy	Figures
cdx4	mesoderm	Fig. S12 from Sun et al., 2018
drl	lateral plate mesoderm ventral mesoderm	Fig. S4 from Sun et al., 2018
fli1	lateral plate mesoderm	Fig. S10 from Sun et al., 2018
gata1a	erythroid lineage cell lateral plate mesoderm	Fig. S6 , Fig. S10 , Fig. S14 from Sun et al., 2018
gata2a	lateral plate mesoderm	Fig. S10 from Sun et al., 2018

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Phenotype

Phenotype resulting from MO2-cftr

Phenotype	Fish	Figures
common myeloid progenitor tal1 expression decreased amount, abnormal	AB + MO2-cftr	Fig. S6 from Sun et al., 2018
common myeloid progenitor myb expression decreased amount, abnormal	AB + MO2-cftr	Fig. S10 from Sun et al., 2018
common myeloid progenitor runx1 expression decreased amount, abnormal	AB + MO2-cftr	Fig. S10 from Sun et al., 2018
erythroid lineage cell hbbe3 expression decreased amount, abnormal	AB + MO2-cftr	Fig. S10 from Sun et al., 2018
erythroid lineage cell gata1a expression decreased amount, abnormal	AB + MO2-cftr	Fig. S6 from Sun et al., 2018

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Phenotype of all Fish created by or utilizing MO2-cftr

Phenotype	Fish	Conditions	Figures
whole organism il1b expression amount, ameliorated	AB + MO1-cftr + MO2-cftr	viral treatment by injection: Pseudomonas phage vB_PaeM_E215, viral treatment by injection: Pseudomonas phage vB_PaeM_E217, viral treatment by injection: Pseudomonas phage vB_PaeP_PYO2, viral treatment by injection: Pseudomonas phage vB_PaeP_DEV	Fig. 4 from Cafora et al., 2019
whole organism decreased life span, ameliorated	AB + MO1-cftr + MO2-cftr	viral treatment by injection: Pseudomonas phage vB_PaeM_E215, viral treatment by injection: Pseudomonas phage vB_PaeM_E217, bacterial treatment by injection: Pseudomonas aeruginosa, viral treatment by injection: Pseudomonas phage vB_PaeP_DEV, viral treatment by injection: Pseudomonas phage vB_PaeP_PYO2, chemical treatment: ciprofloxacin	Fig. 5 from Cafora et al., 2019
whole organism tnfa expression increased amount, abnormal	AB + MO1-cftr + MO2-cftr	control	Fig. 4 from Cafora et al., 2019
heart looping disrupted, abnormal	AB + MO1-cftr + MO2-cftr	standard conditions	Fig. 1 from Cafora et al., 2020
defense response to bacterium decreased efficacy, abnormal	AB + MO1-cftr + MO2-cftr	bacterial treatment by injection: Pseudomonas aeruginosa	Fig. 2 , Fig. 3 from Cafora et al., 2019

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Citations

Include unpublished citations

Cafora, M., Poerio, N., Forti, F., Loberto, N., Pin, D., Bassi, R., Aureli, M., Briani, F., Pistocchi, A., Fraziano, M. (2022) Evaluation of phages and liposomes as combination therapy to counteract Pseudomonas aeruginosa infection in wild-type and CFTR-null models. Frontiers in microbiology. 13:979610
Cafora, M., Brix, A., Forti, F., Loberto, N., Aureli, M., Briani, F., Pistocchi, A. (2020) Phages as immunomodulators and their promising use as anti-inflammatory agents in a cftr loss-of-function zebrafish model. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society. 20(6):1046-1052
Cafora, M., Forti, F., Briani, F., Ghisotti, D., Pistocchi, A. (2020) Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos. Journal of visualized experiments : JoVE. (159):
Cafora, M., Deflorian, G., Forti, F., Ferrari, L., Binelli, G., Briani, F., Ghisotti, D., Pistocchi, A. (2019) Phage therapy against Pseudomonas aeruginosa infections in a cystic fibrosis zebrafish model. Scientific Reports. 9:1527
Sun, H., Wang, Y., Zhang, J., Chen, Y., Liu, Y., Lin, Z., Liu, M., Sheng, K., Liao, H., Tsang, K.S., Zhang, X., Jiang, X., Xu, W., Mao, M., Chan, H.C. (2018) CFTR mutation enhances Dishevelled degradation and results in impairment of Wnt-dependent hematopoiesis. Cell Death & Disease. 9:275
Phennicie, R.T., Sullivan, M.J., Singer, J.T., Yoder, J.A., and Kim, C.H. (2010) Specific Resistance to Pseudomonas aeruginosa Infection in Zebrafish is Mediated by the Cystic Fibrosis Transmembrane Conductance Regulator. Infection and Immunity. 78(11):4542-4550

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