ZFIN ID: ZDB-LAB-991015-4
Jesuthasan Lab
PI/Director: Jesuthasan, Suresh
Contact Person: Jesuthasan, Suresh
Email: suresh.jesuthasan@nrp.a-star.edu.sg
URL: http://www.tll.org.sg/res/dn_main.asp
Address: A*Star/Duke-NUS Neuroscience Research Partnership 04-13B Proteos Biopolis Drive Singapore 138673
Country: Singapore
Phone: 65-6586-9538
Fax:
Line Designation: sq


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 17 genomic features


STATEMENT OF RESEARCH INTERESTS
FEAR
We use the alarm response as a paradigm to study the neural basis of innate fear.

The alarm response in fish was first noticed in the 1930’s by Karl von Frisch. He observed that injury to one minnow caused a fright reaction in other members of the school. He demonstrated that the skin contained substances, termed Schreckstoff, which act via the olfactory system to trigger a state of fear. The fish change their swimming behavior dramatically - either darting or freezing - in response to this alarm pheromone.

Subsequent experiments by other scientists established that many freshwater fish species have this response. All the classical hallmarks of fear, including physiological changes such as increase in blood cortisol levels, can be triggered by Schreckstoff.

There are a number of projects in the lab aimed at understanding how different levels of fear are generated. As a starting point, we have embarked on purification and identification of Schreckstoff. We have also carried out calcium imaging of the brain to identify neurons involved. A preliminary genetic screen was carried out in Tuebingen, and this has led to the identification of potential mutations affecting the response. In collaboration with S. Parinov and A. Emelyanov, we are using the LexPR enhancer trap system to manipulate neurons that are potentially involved in the response.

In addition to innate fear, we also carry out experiments using learnt fear, using a larval conditioning paradigm. These experiments are being carried out in collaboration with the laboratory of Trevor Penney, who is in the psychology department in NUS.


LAB MEMBERS
Mathuru, Ajay Post-Doc Min, Aletheia Lee Graduate Student Kibat, Caroline Technical Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
Kibat, C., Krishnan, S., Ramaswamy, M., Baker, B.J., Jesuthasan, S. (2016) Imaging voltage in zebrafish as a route to characterizing a vertebrate functional connectome: promises and pitfalls of genetically encoded indicators. Journal of neurogenetics. 30:80-8
Krishnan, S., Mathuru, A.S., Kibat, C., Rahman, M., Lupton, C.E., Stewart, J., Claridge-Chang, A., Yen, S.C., Jesuthasan, S. (2014) The Right Dorsal Habenula Limits Attraction to an Odor in Zebrafish. Current biology : CB. 24(11):1167-75
Cheng, R.K., Jesuthasan, S.J., and Penney, T.B. (2014) Zebrafish forebrain and temporal conditioning. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 369(1637):20120462
Schirmer, A., Jesuthasan, S., and Mathuru, A.S. (2013) Tactile stimulation reduces fear in fish. Frontiers in behavioral neuroscience. 7:167
Tan, S.J., Kee, M.Z., Mathuru, A.S., Burkholder, W.F., and Jesuthasan, S.J. (2013) A microfluidic device to sort cells based on dynamic response to a stimulus. PLoS One. 8(11):e78261
Mathuru, A.S., and Jesuthasan, S. (2013) The medial habenula as a regulator of anxiety in adult zebrafish. Frontiers in neural circuits. 7:99
Mathuru, A.S., Kibat, C., Cheong, W.F., Shui, G., Wenk, M.R., Friedrich, R.W., and Jesuthasan, S. (2012) Chondroitin fragments are odorants that trigger fear behavior in fish. Current biology : CB. 22(6):538-544
Jesuthasan, S. (2012) Fear, anxiety, and control in the zebrafish. Developmental Neurobiology. 72(3):395-403
Kalueff, A.V., Stewart, A.M., Kyzar, E.J., Cachat, J., Gebhardt, M., Landsman, S., Robinson, K., Maximino, C., Herculano, A.M., Jesuthasan, S., Wisenden, B., Bally-Cuif, L., Lange, M., Vernier, P., Norton, W., Tierney, K., Tropepe, V., and Neuhauss, S. (2012) Time to recognize zebrafish ‘affective’ behavior. Behaviour. 149:1019-1036
Cheng, R.K., Jesuthasan, S., and Penney, T.B. (2011) Time for zebrafish. Frontiers in Integrative Neuroscience. 5:40
Lee, A., Mathuru, A.S., Teh, C., Kibat, C., Korzh, V., Penney, T.B., and Jesuthasan, S. (2010) The habenula prevents helpless behavior in larval zebrafish. Current biology : CB. 20(24):2211-2216
Sheng, D., Qu, D., Kwok, K.H., Ng, S.S., Lim, A.Y., Aw, S.S., Lee, C.W., Sung, W.K., Tan, E.K., Lufkin, T., Jesuthasan, S., Sinnakaruppan, M., and Liu, J. (2010) Deletion of the WD40 domain of LRRK2 in Zebrafish causes Parkinsonism-like loss of neurons and locomotive defect. PLoS Genetics. 6(4):e1000914
Hendricks, M., and Jesuthasan, S. (2009) PHR regulates growth cone pausing at intermediate targets through microtubule disassembly. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(20):6593-6598
Jesuthasan, S.J., and Mathuru, A.S. (2008) The alarm response in zebrafish: innate fear in a vertebrate genetic model. Journal of neurogenetics. 22(3):211-228
Hendricks, M., Mathuru, A.S., Wang, H., Silander, O., Kee, M.Z., and Jesuthasan, S. (2008) Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation. Molecular and Cellular Neuroscience. 37(2):271-283
Hendricks, M., and Jesuthasan, S. (2007) Asymmetric innervation of the habenula in zebrafish. The Journal of comparative neurology. 502(4):611-619
Hendricks, M., and Jesuthasan, S. (2007) Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development. Neural Development. 2(1):6
Etard, C., Behra, M., Ertzer, R., Fischer, N., Jesuthasan, S., Blader, P., Geisler, R., and Strähle, U. (2005) Mutation in the delta-subunit of the nAChR suppresses the muscle defects caused by lack of Dystrophin. Developmental dynamics : an official publication of the American Association of Anatomists. 234(4):1016-1025
Feng, B., Bulchand, S., Yaksi, E., Friedrich, R.W., and Jesuthasan, S.J. (2005) The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not essential for axon targeting or amino acid detection. BMC Neuroscience. 6:46
Le Guyader, S., Maier, J., and Jesuthasan, S. (2005) Esrom, an ortholog of PAM (protein associated with c-myc), regulates pteridine synthesis in the zebrafish. Developmental Biology. 277(2):378-386
D'Souza, J., Hendricks, M., Le Guyader, S., Subburaju, S., Grunewald, B., Scholich, K., and Jesuthasan, S. (2005) Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc). Development (Cambridge, England). 132(2):247-256
Hendricks, M., and Jesuthasan, S. (2004) Form and Function in the Zebrafish Nervous System. In Fish Development and Genetics - the Zebrafish and Medaka Models. Gong, Z. and Korh, V., Eds. Book series of "Molecular Aspects of Fish and Marine Biology". 2
Wagle, M., Grunewald, B., Subburaju, S., Barzaghi, C., Le Guyader, S., Chan, J., and Jesuthasan, S. (2004) EphrinB2a in the zebrafish retinotectal system. Journal of neurobiology. 59(1):57-65
Wagle, M. and Jesuthasan, S. (2003) Baculovirus-mediated gene expression in zebrafish. Mar. Biotechnol.. 5(1):58-63
Feng, B., Schwarz, H., and Jesuthasan, S. (2002) Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres. Experimental cell research. 279(1):14-20
Jesuthasan, S. (2002) Genetics and development: zebrafish in the spotlight. Science (New York, N.Y.). 297(5586):1484-1485
Jesuthasan, S. and Subburaju, S. (2002) Gene transfer into zebrafish by sperm nuclear transplantation. Developmental Biology. 242(2):88-95
Le Guyader, S. and Jesuthasan, S. (2002) Analysis of xanthophore and pterinosome biogenesis in zebrafish using methylene blue and pteridine autofluorescence. Pigment Cell Res.. 15(1):27-31
Jesuthasan, S. (1998) Furrow-associated microtubule arrays are required for the cohesion of zebrafish blastomeres following cytokinesis. Journal of Cell Science. 111:3695-3703
Jesuthasan, S. (1997) Neural crest cell migration in the zebrafish can be mimicked by inert objects: mechanism and implication of latex bead movement in embryos. The Journal of experimental zoology. 277(6):425-434
Strähle, U., Jesuthasan, S., Blader, P., Garcia-Villalba, P., Hatta, K., and Ingham, P.W. (1997) one-eyed pinhead is required for development of the ventral midline of the zebrafish (Danio rerio) neural tube. Genes and function. 1:131-148
Jesuthasan, S. and Strähle, U. (1997) Dynamic microtubules and specification of the zebrafish embryonic axis. Current biology : CB. 7(1):31-42
Jesuthasan, S. (1996) Contact inhibition collapse and pathfinding of neural crest cells in the zebrafish trunk. Development (Cambridge, England). 122:381-389
Strähle, U. and Jesuthasan, S. (1993) Ultraviolet irradiation impairs epiboly in zebrafish embryos: evidence for a microtubule-dependent mechanism of epiboly. Development (Cambridge, England). 119:909-919