ZFIN ID: ZDB-LAB-181219-1
Ninov Lab
PI/Director: Ninov, Nikolay
Contact Person: Ninov, Nikolay
Email: nikolay.ninov@tu-dresden.de
URL: https://tu-dresden.de/cmcb/crtd/forschungsgruppen/crtd-forschungsgruppen/ninov
Address: CRTD / Center for Regenerative Therapies Dresden - Cluster of Excellence, Fetscherstraße 105 01307 Dresden
Country: Germany
Phone: +49 (0)351 458 82314
Fax: +49 351 458-82059
Line Designation: tud


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 6 genomic features


STATEMENT OF RESEARCH INTERESTS
TOWARDS IN VIVO REGENERATION OF BETA-CELLS AS A FUTURE THERAPY FOR T1D

The pancreatic β-cells are the key metabolic sensors and effectors for insulin release, which is the only hormone known to lower blood glucose concentrations. Type 1 diabetes mellitus is an autoimmune disease characterized by the destruction of beta-cells in the pancreatic islets, leading to insulin deficiency and hyperglycemia. In contrast, type 2 diabetes commences with insulin resistance followed by beta cell loss and hyperglycemia. Restoring functional β-cell mass is recognized as a promising therapeutic avenue towards normalizing glycemic control in both type 1 and 2 diabetics. Thus, our research goal is to understand the molecular and cellular events required for pancreatic β-cell regeneration, and apply this knowledge towards the development of cell replacement therapies for diabetes.


LAB MEMBERS
Akhtar, Nadeem Graduate Student Delgadillo Silva, Luis Fernando Graduate Student Janjuha, Sharan Graduate Student
Kamel, Margrit Graduate Student Palme, Silvia Administrative Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Ellis, J.L., Evason, K.J., Zhang, C., Fourman, M.N., Liu, J., Ninov, N., Delous, M., Vanhollebeke, B., Fiddes, I., Otis, J.P., Houvras, Y., Farber, S.A., Xu, X., Lin, X., Stainier, D.Y.R., Yin, C. (2022) A missense mutation in the proprotein convertase gene furinb causes hepatic cystogenesis during liver development in zebrafish. Hepatology communications. 6(11):3083-3097
Singh, S.P., Chawla, P., Hnatiuk, A., Kamel, M., Silva, L.D., Spanjaard, B., Eski, S.E., Janjuha, S., Olivares-Chauvet, P., Kayisoglu, O., Rost, F., Bläsche, J., Kränkel, A., Petzold, A., Kurth, T., Reinhardt, S., Junker, J.P., Ninov, N. (2022) A single-cell atlas of de novo β-cell regeneration reveals the contribution of hybrid β/δ-cells to diabetes recovery in zebrafish. Development (Cambridge, England). 149(2)
Delgadillo-Silva, L.F., Akhtar, M.N., Taşöz, E., Ninov, N. (2021) Simultaneous Calcium Imaging and Glucose Stimulation in Living Zebrafish to Investigate In Vivo β-Cell Function. Journal of visualized experiments : JoVE. (175):
Mousavy Gharavy, S.N., Owen, B.M., Millership, S.J., Chabosseau, P., Pizza, G., Martinez-Sanchez, A., Tasoez, E., Georgiadou, E., Hu, M., Fine, N.H.F., Jacobson, D.A., Dickerson, M.T., Idevall-Hagren, O., Montoya, A., Kramer, H., Mehta, Z., Withers, D.J., Ninov, N., Gadue, P.J., Cardenas-Diaz, F.L., Cruciani-Guglielmacci, C., Magnan, C., Ibberson, M., Leclerc, I., Voz, M., Rutter, G.A. (2021) Sexually dimorphic roles for the type 2 diabetes-associated C2cd4b gene in murine glucose homeostasis. Diabetologia. 64(4):850-864
Gillotay, P., Shankar, M., Haerlingen, B., Sema Elif, E., Pozo-Morales, M., Garteizgogeascoa, I., Reinhardt, S., Kränkel, A., Bläsche, J., Petzold, A., Ninov, N., Kesavan, G., Lange, C., Brand, M., Lefort, A., Libert, F., Detours, V., Costagliola, S., Singh, S.P. (2020) Single-cell transcriptome analysis reveals thyrocyte diversity in the zebrafish thyroid gland. EMBO reports. 21(12):e50612
Deville, S.S., Delgadillo Silva, L.F., Vehlow, A., Cordes, N. (2020) c-Abl Tyrosine Kinase Is Regulated Downstream of the Cytoskeletal Protein Synemin in Head and Neck Squamous Cell Carcinoma Radioresistance and DNA Repair. International Journal of Molecular Sciences. 21(19):
Singh, S.P., Ninov, N. (2020) Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2128:159-179
Salem, V., Silva, L.D., Suba, K., Georgiadou, E., Neda Mousavy Gharavy, S., Akhtar, N., Martin-Alonso, A., Gaboriau, D.C.A., Rothery, S.M., Stylianides, T., Carrat, G., Pullen, T.J., Singh, S.P., Hodson, D.J., Leclerc, I., Shapiro, A.M.J., Marchetti, P., Briant, L.J.B., Distaso, W., Ninov, N., Rutter, G.A. (2019) Leader β-cells coordinate Ca2+ dynamics across pancreatic islets in vivo. Nature metabolism. 1:615-629
Dickmeis, T., Feng, Y., Mione, M.C., Ninov, N., Santoro, M., Spaink, H.P., Gut, P. (2019) Nano-Sampling and Reporter Tools to Study Metabolic Regulation in Zebrafish. Frontiers in cell and developmental biology. 7:15
Delgadillo-Silva, L.F., Tsakmaki, A., Akhtar, N., Franklin, Z.J., Konantz, J., Bewick, G.A., Ninov, N. (2019) Modelling pancreatic β-cell inflammation in zebrafish identifies the natural product wedelolactone for human islet protection. Disease models & mechanisms. 12(1):
Singh, S.P., Janjuha, S., Chaudhuri, S., Reinhardt, S., Kränkel, A., Dietz, S., Eugster, A., Bilgin, H., Korkmaz, S., Zararsız, G., Ninov, N., Reid, J.E. (2018) Machine learning based classification of cells into chronological stages using single-cell transcriptomics. Scientific Reports. 8:17156
Janjuha, S., Pal Singh, S., Ninov, N. (2018) Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets. Journal of visualized experiments : JoVE. (137)
Spanjaard, B., Hu, B., Mitic, N., Olivares-Chauvet, P., Janjuha, S., Ninov, N., Junker, J.P. (2018) Simultaneous lineage tracing and cell-type identification using CRISPR-Cas9-induced genetic scars. Nature Biotechnology. 36(5):469-473
Janjuha, S., Singh, S.P., Tsakmaki, A., Mousavy-Gharavy, N., Murawala, P., Konantz, J., Birke, S., Hodson, D.J., Rutter, G., Bewick, G., Ninov, N.N. (2018) Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish. eLIFE. 7
So, J., Khaliq, M., Evason, K., Ninov, N., Martin, B.L., Stainier, D.Y.R., Shin, D. (2017) Wnt/β-catenin signaling controls intrahepatic biliary network formation in zebrafish by regulating Notch activity. Hepatology (Baltimore, Md.). 67(6):2352-2366
Choi, T.Y., Khaliq, M., Tsurusaki, S., Ninov, N., Stainier, D.Y.R., Tanaka, M., Shin, D. (2017) Bmp Signaling Governs Biliary-Driven Liver Regeneration in Zebrafish via Tbx2b and Id2a. Hepatology (Baltimore, Md.). 66(5):1616-1630
Singh, S.P., Janjuha, S., Hartmann, T., Kayisoglu, Ö., Konantz, J., Birke, S., Murawala, P., Alfar, E.A., Murata, K., Eugster, A., Tsuji, N., Morrissey, E.R., Brand, M., Ninov, N. (2017) Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth. Nature communications. 8:664
Kamel, M., Ninov, N. (2017) Catching new targets in metabolic disease with a zebrafish. Current opinion in pharmacology. 37:41-50
Ahmed Alfar, E., Kirova, D., Konantz, J., Birke, S., Mansfeld, J., Ninov, N. (2017) Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity. Scientific Reports. 7:3994
Ninov, N., Yun, M.H. (2015) Current advances in tissue repair and regeneration: the future is bright. Regeneration (Oxford, England). 2:84-91
Tsuji, N., Ninov, N., Delawary, M., Osman, S., Roh, A.S., Gut, P., Stainier, D.Y. (2014) Whole Organism High Content Screening Identifies Stimulators of Pancreatic Beta-Cell Proliferation. PLoS One. 9:e104112
Choi, T.Y., Ninov, N., Stainier, D.Y., and Shin, D. (2014) Extensive Conversion of Hepatic Biliary Epithelial Cells to Hepatocytes after Near Total Loss of Hepatocytes in Zebrafish. Gastroenterology. 146(3):776-88
Ninov, N., Hesselson, D., Gut, P., Zhou, A., Fidelin, K., and Stainier, D.Y. (2013) Metabolic Regulation of Cellular Plasticity in the Pancreas. Current biology : CB. 23(13):1242-50
Ninov, N., Borius, M., and Stainier, D.Y. (2012) Different levels of Notch signaling regulate quiescence, renewal and differentiation in pancreatic endocrine progenitors. Development (Cambridge, England). 139(9):1557-1567
Delous, M., Yin, C., Shin, D., Ninov, N., Debrito Carten, J., Pan, L., Ma, T.P., Farber, S.A., Moens, C.B., and Stainier, D.Y. (2012) sox9b Is a Key Regulator of Pancreaticobiliary Ductal System Development. PLoS Genetics. 8(6):e1002754