Figure 7

Figure 7 Knockdown of wbp11 causes spinal defects in zebrafish. (A–F) Bright field microscopy observation of spinal morphology in zebrafish larvae. Red scale bar: 100 μm (applies to (A,B)); red scale bar: 50 μm (applies to (C–F)). (G–J) Whole-mount in situ hybridization of zebrafish larvae at 24 hpf using the shha probe to assess spinal development. Scale bar: 250 μm (applies to (G,H)); scale bar: 125 μm (applies to (I,J)). (K) Heatmap analysis of spine related differentially expressed genes (DEGs). Blue indicates downregulated genes, and red indicates upregulated genes. (L) FPKM values of representative spine-related genes sox9a and fgf23 (n = 3; biologically independent experiments, each performed with 50 pooled embryos per group. Data are presented as mean ± SEM. *p < 0.05, ***p < 0.001). (M) qPCR analysis of transcript levels of representative spine-related genes sox9a and fgf23 (n = 3; biologically independent experiments, each performed with 50 pooled embryos per group. Data are presented as mean ± SEM. ***p < 0.001).