Figure 3.
- ID
- ZDB-IMAGE-260902-3
- Publication
- Gan et al., 2026 - COMMD9-regulated endothelial cell abnormality-induced hypercoagulability is associated with Budd-Chiari syndrome
- All Figures
- Figures for Gan et al., 2026
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Figure 3.
COMMD9 knockdown elevates cell adhesion and inhibits migration and tube formation in ECs. (A) Western blot analysis of COMMD9 expression in four types of human vascular ECs and smooth muscle cells. (B) Cell adhesion assay of HUVECs and HHSECs after transfection with siNC or siRNA targeting COMMD9 (siRNA-1 or siRNA-2). (C) Western blot analysis of LAMB1, VWF (S) (cell supernatants of trichloroacetic acid precipitates were subjected to immunoblotting to measure secreted VWF), PECAM1, and VCAM1 in HUVECs and HHSECs with COMMD9 knockdown. (D) Migration assay of HUVECs and HHSECs after transfection with siNC or siRNA targeting COMMD9. (E, F) Tube formation assays of transfected HUVECs and HHSECs. Scale bar, 100 μm. N = 3 biological replicates. The results are shown as mean ± SD, ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05.