Fig S11

Fig S11 tgfb3 is expressed in subsets of fibroblasts and in close contact with ERGs and growing axons.
(A) UMAP of fibroblasts subset showing 7 clusters of cells. (B) Top 10-ranked gene analysis showing differential expressing genes among clusters. (C) UMAP showing genes used for annotation and tentative cluster names. (D) Feature plot showing expression pattern of tgfb3. Arrows point enriched clusters for the gene. (E) Single-Stacks of the lesion site at the level of the spinal cord showing tgfb3-expressing fibroblasts (arrow). (F) Live imaging showing that fibroblasts (green, yellow arrows) are in the vicinity of motor neuron progenitors (magenta, cyan arrows). (G) Live imaging showing that fibroblasts (green, yellow arrows) are in close contact with growing axons (magenta, cyan arrows). Yellow dotted lines mark the spinal cord. Scale bars: 50 µm.