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Fig S3

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ZDB-IMAGE-260821-24
Publication
Docampo-Seara et al., 2026 - The microglia-derived protein sema4ab attenuates regenerative neurogenesis after spinal cord injury in zebrafish
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Figures for Docampo-Seara et al., 2026
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Figure Caption

Fig S3 HCR-FISH method control and detection of sema4ab expression in neutrophils.
(A) HCR-FISH for the negative control gene (Drosophila BicoidXA) shows non-specific labeling in some blood vessels, but not in the spinal cord in uninjured and injured larvae. (B) Multiplexed HCR-FISH at 6 and 24 hpl shows that some sema4ab+ cells express mpx:GFP (white arrows: double-positive cells; green arrows: mpx:GFP+ only; magenta arrows: sema4ab+ only). Scale bars: 100 µm (A, B), 10 µm (inset). Data files for graphs available in S3 Data.

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