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Fig. 4

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Tsuji et al., 2026 - Neu1 deficiency is associated with reduced nicotine responsiveness and dopamine D3 receptor downregulation in zebrafish
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Fig. 4

Nicotine-induced neuronal activation in polysialic acid-positive neurons in the hypothalamus. A Representative immunohistochemical images of the hypothalamus from WT and neu1-KO zebrafish. Fluorescence intensities are expressed relative to the WT value. n = 3. B Double immunofluorescence staining for c-Fos (red) and PSA (green) in WT zebrafish following Nco exposure, with merged images showing spatial association between neuronal activation and PSA-positive regions. Nuclei were counterstained with DAPI. PTN, posterior tuberal nucleus. HC, hypothalamic periventricular complex. Scale bars, 100 µm

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