Fig. 2

Fig. 2

Behavioral effects of nicotine exposure in WT and neu1-knockout zebrafish. A Schematic illustration of the behavioral tank used for side-view swimming analysis. The water column was vertically divided into upper and lower layers for the spatial analysis of swimming behavior. B Survival rates of WT and neu1-KO zebrafish exposed to increasing concentrations of Nco (0, 2.5, 7.5, and 10 mg/L) during a span of 10 min. C Representative heatmaps of swimming trajectories for WT and neu1-KO zebrafish exposed to different Nco concentrations. Color intensity indicates the density of swimming activity, with warmer colors representing higher occupancy. D Total swimming distance traveled during the behavioral recording period. E Swimming distance in the upper layer of the tank. F Time spent swimming in the upper layer. G Duration of excited swimming behavior. Different letters indicate statistically significant differences between the groups. n = 6