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Biswas et al., 2026 - Canalization of neural dynamics by δ-protocadherins in the developing zebrafish optic tectum
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Fig. S2 Generation of promoterless pcdh19 allele.
A Schematic of the zebrafish pcdh19 gene, with exons shown in purple. Pins show the sites of CRISPR/Cas9 target sites. Primers used for PCR-based screening are shown, as are the expected sizes of the PCR products. B Genotyping of mutant in-crosses using F1, F2 and R primers, which distinguish wild type, heterozygous and homozygous mutant larvae. C Western blot showing the absence of Pcdh19 protein in both our previously published mutant line and in the promoterless line presented here.

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