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Fig. 5 Expression of key genes and proteins in the Amh/Bmpr2a pathway in WT female zebrafish ovaries after 150-day exposure to neburon. (A) Transcriptional levels of amh, bone morphogenetic protein receptor 2a (bmpr2a), and bmpr2b in ovaries (n = 6 samples/group) compared to control fish. (B) Protein levels of Bmpr2, phosphorylated small mother against decapentaplegic (pSmad1), and Smad1 in ovaries (n = 3 samples/group) compared to control fish. (C) The representative ovarian sections were labeled with pSmad1 using immunostaining. Areas in brown indicated positive staining signal. The positive signal area was magnified in the lower panel, and the granulosa cells were indicated by the arrow. Scale bars, 200 μm (top panel) and 100 μm (bottom panel), respectively. (D) The proportion of pSmad1-positive (pSmad1+) area to total follicles area in ovarian sections (n = 4 samples/group). (E) Protein levels of Fshr in ovaries (n = 3 samples/group) of control and neburon-exposed fish. Data are presented as the mean ± SE. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by posthoc Duncan’s test. Different letters on the bars represent statistically significant differences at p < 0.05 between groups. The data presented in Figure 5A,B,D,E are also displayed in Excel Table S19, S20, S21 and S22, respectively.