ZFIN Image: Liu et al., 2026, Figure 2

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Figure Caption

Figure 2

Co-infection experiments show distinct spatial colonization patterns of MAC 101 and Mma20 bacteria. (A) Schematic of the experimental workflow. Germ-free (GF) and conventionalized (CONVD) zebrafish larvae derived from Casper fish line were immersed in egg water containing mWasabi-labeled M. avium (MAC 101) and DsRed -labeled M. marinum (Mma20) at the same time for 2.5 days. (B) Representative fluorescence images show that MAC 101 preferentially localizes to the posterior part of gut and Mma20 preferentially localizes to the anterior part of gut in CONVD larvae, whereas this regional bias is lost in GF conditions. MAC 101 bacteria are shown in cyan; Mma20 bacteria are shown in magenta. Scale bar: 100 µm. The data from GF (n=14) group and CONVD (n=14) group are based on two independent experiments. Statistical significant difference was determined by one-way ANOVA, red arrows point to the median, ns, non-significant, *, P < 0.05, **, P < 0.01, ***, P < 0.001.

Acknowledgments

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