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Fig. 6 - Supplemental 5 Spatial analysis of lipid droplet (LD) size along the anterior-posterior axis in foxp1a and foxp1b mutants following high-fat diet (HFD) challenge. (a) Representative Nile Red fluorescence montages of the subcutaneous adipose depot in wild-type and foxp1aed116 fish under control diet (CD) and HFD conditions. (b) Representative Nile Red montages of wild-type and foxp1bed125 fish under CD and HFD conditions. (c) Mean LD diameter (µm) per 200 µm stratum along the anterior-posterior axis for wild-type (grey) and foxp1aed116 mutants (teal) under CD (light) and HFD (dark). Lines represent LOESS fits with shaded 95% confidence intervals; dots represent individual stratum means per fish. Strata are numbered from anterior (1) to posterior (15). (d) Individual fish traces for each group in the foxp1a experiment. Thin lines represent individual fish; thick lines with shading represent group means. Sample sizes are indicated. (e) Mean LD diameter per stratum for wild-type (grey) and foxp1bed125 mutants (pink/red) under CD and HFD, plotted as in (c). (f) Individual fish traces for each group in the foxp1b experiment, plotted as in (d).