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Publication: Wafer et al., 2026 - A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish
All Figures: Figures for Wafer et al., 2026

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Fig. 1 Experimental workflow. (1) Identification of candidate morphology genes from bulk RNA-Seq of human subcutaneous adipose tissue (SAT), comparing genes enriched in small versus large adipocytes (Honecker et al.). (2) Filtering for expression in adipose stem and progenitor cells using sn/scRNA-Seq of 16 white adipose tissue (WAT) cell types (Emont et al.). (3) Gene Ontology (GO) term enrichment analysis to focus on genes involved in development and differentiation processes. (4) Prioritisation of candidate genes based on protein conservation to zebrafish and expression dynamics during adipocyte progenitor differentiation. (5) In vivo F0 CRISPR screen in zebrafish using multiple guide RNAs (gRNAs) per gene to quantify lipid droplet morphology (hyperplastic versus hypertrophic).

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