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Fig. 6
Chemical proteomics identified the binding targets of OGP1-1 in RAW 264.7 cells. (A) General workflow for Chemical proteomics profiling of OGP1-1 targets. (B) Labelling for OGP1-1 probe (OGP1-1-P) in RAW 264.7 cells. (C) The competition of proteins labeled with OGP1-1-P by excess OGP1-1. (D) Scatter plot depicting the differential enrichment of proteins. TAK1 is the potential target (Top1 in fold change of OGP1-1-P/Compete). (E) Venn digraph displaying the amount and overlap of proteins both enriched in those two groups. (F) Scatter diagram for gene ontology enrichment analysis of differential proteins. (G) The binding instant (Kd) between OGP1-1 and TAK1 determined by MST. (H) CETSA-WB and statistical analysis of the protein levels, Data were expressed as mean ± SD; **