Fig. 5

Fig. 5

Effect of OGP1-1 on CuSO₄-induced inflammation in zebrafish model. (A) Representative green-fluorescent neutrophils from different groups (bar = 200 μm). The white arrow indicated the inflammatory cells migrate from circulation to injured lateral line nervous. (B) Quantitative analysis of the number of macrophages in the zebrafish inflammatory models before and after the administration of OGP1-1 and ibuprofen, respectively. Data were expressed as mean ± SEM; **P < 0.01 vs. Control group; ## P < 0.01 vs. CuSO₄ group, n = 10 larvae each group. The effects of OGP1-1 on the expression levels of inflammation-related genes, il-1β (C), cox-2 (D), tnf-α (E), and nos2b (F), in zebrafish. Gene mRNA expression is shown as the relative expression by fold compared to the control. Data were expressed as mean ± SD; **P < 0.01 vs. Control group; ## P < 0.01 vs. CuSO₄ group, n = 50 juvenile zebrafish per group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)