Figure 3.

Figure 3.

CERKL deficiency leads to lipid accumulation and PC reduction in RPE-1 cells. (A) Western blot analysis of CERKL expression in the RPE-1 cells after siRNA knockdown for 72 hours. (B) Quantitative analysis of the Western blot data. At least three independent experiments were performed and quantified. The results are shown as mean ± SD. ***, P < 0.001. (C) Real-time PCR analysis of CERKL mRNA expression in RPE-1 cells after siRNA knockdown for 72 hours. ***, P < 0.001. (D) BODIPY staining and Perilipin-2 immunostaining in RPE-1 cells after infection with siCERKL and its corresponding control followed by OA or BSA treatment. Scale bars = 10 µm. (E) BODIPY staining density per cell number as quantified by ImageJ software (n = 6 fields/group). (F) Nile red staining in RPE-1 cells after NC and siCERKL transfection with or without CERKL-GFP overexpression. Scale bars = 10 µm. (G) Nile red staining density per cell of vectors overexpression as quantified by ImageJ software. At least 30 cells were calculated from three independent experiments. (H) The triglyceride levels in NC and siCERKL groups. **, P < 0.01. (I) The PC levels in NC and siCERKL groups. *, P < 0.05.