Fig. 8.

Fig. 8.

Motor activity of microbiome-depleted and SIHUMIx-inoculated zebrafish larvae exposed to azoxystrobin across various stimulus modalities. A and B) Motor activity (y-axis) of SIHUMIx-inoculated zebrafish larvae exposed to 0.4% DMSO (n = 42, gray) is compared with A) SIHUMIx-inoculated zebrafish larvae exposed to 1.68 µM azoxystrobin (n = 46, yellow) and B) microbiome-depleted zebrafish larvae exposed to 1.68 µM azoxystrobin (n = 47, blue). Data are median ± 95% CI. E) A subset of VAMR line plots highlighting motor activity during the visual motor response (VMR2) endpoint. Raincloud plots quantify motor activity and habituation metrics across stimulus modalities. Motor responses are shown under C) illuminated and D) non-illuminated (dark) conditions, F) during inter-endpoint intervals, H) during inter-bout intervals, G) during acoustic startle habituation, and I) as total motor activity across all 5 habituation bouts calculated as the acoustic startle habituation sum (ASHsum). Cohorts from both colonization statuses were exposed to DMSO, 0.6 µM, or 1.68 µM azoxystrobin; statistical significance was assessed relative to the SIHUMIx-inoculated DMSO group. Horizontal dotted lines indicate median motor activity of vehicle-exposed SIHUMIx-inoculated larvae. Numbers above the rainclouds represent Benjamini–Hochberg adjusted P-values (gray: P ≥ 0.05, black: P < 0.05; 2-sample bootstrapping test). J) A heatmap of P-values comparing median responses to the vehicle control across all endpoints (y-axis) and experimental groups (x-axis). Heatmap colors represent P-value comparisons to the colonized control group, with gray representing no significant difference, and red and blue indicating increased and reduced motor activity, respectively. Sample sizes: SIHUMIx-inoculated DMSO (n = 42), SIHUMIx-inoculated 0.6 µM (n = 45), SIHUMIx-inoculated 1.68 µM (n = 46), microbiome-depleted DMSO (n = 45), microbiome-depleted 0.6 µM (n = 47), and microbiome-depleted 1.68 µM (n = 47).