ZFIN Image: Wray et al., 2026, Fig. 2.

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Figure Caption

Fig. 2.

Zebrafish derivation experimental design. Cohort generation and exposure design. At 0 dpf, zebrafish embryos were collected and underwent microbiome depletion (n = 30 embryos/flask). Microbiome-depleted larvae were maintained under sterile conditions, whereas a subset of embryos was inoculated with SIHUMIx on day 1 to generate the SIHUMIx-inoculated cohort. At 4 dpf, larvae in both cohorts were statically exposed to azoxystrobin (0.6 or 1.68 μM) or the vehicle control (0.4% DMSO). At 5 dpf, larvae were plated for behavior assessment with the Visual Acoustic Motor Response (VAMR) assay, with remaining larvae collected for 16S rRNA sequencing or metabolomics analyses. Created in BioRender (Wray 2026b). https://BioRender.com/ymgg921.

Acknowledgments

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