Figure 2.

Figure 2.

Claudin-19 plays an essential role in maintaining the outer BRB. (A) Real-time barrier-resistant measurement (2000 Hz) of primary porcine RPE cells treated with 25-nM scrambled siRNA or claudin-19 siRNA or were left untransfected. Only final points were quantified. Data are mean ± SD (n = 5). ****P < 0.0001 (ordinary one-way ANOVA with Tukey's test). (B) Western blot analysis of porcine RPE treated for 24, 48, and 72 hours with 25-nM scrambled siRNA or claudin-19 siRNA. β-Actin was used as a loading control. Data are mean ± SD (n = 5). ****P < 0.0001 (ordinary one-way ANOVA with Tukey's test). (C) Immunofluorescence staining of claudin-19 (green) and ZO-1 (red) in porcine RPE cells. Scale bar: 25 µm. (D) mRNA expression of claudin-1, claudin-3, claudin-5, claudin-11, and claudin-19 after 72-hour treatment with 25-nM scrambled siRNA or claudin-19 siRNA. Data are mean ± SD (n = 3). ***P < 0.0001 (two-way ANOVA with Tukey's test); ns, not significant.