ZFIN Image: Anderson et al., 2026, Figure 4

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Figure 4

Development of notochord tumors in zebrafish. (A) Alcian Blue staining of non-Tg larvae at 6 dpf. (B) Coronal section of non-Tg larvae tail at 6 dpf with H&E staining. (C) Sagittal section of non-Tg larvae tail at 6 dpf with H&E staining. (D) Alcian Blue staining of Tg larvae at 6 dpf. The red circles denote notochordal neoplasms. Black arrows indicate the notochord in panels (A,D). (E) Coronal section of Tg larvae tail at 6 dpf with H&E staining. (F) Sagittal sections of Tg larvae tail at 6 dpf with H&E staining. These data show that expression of EWSR1::FLI1 in col2a1a-expressing cells disrupts normal notochord organization and induces dysplastic notochordal growths during early zebrafish development, as demonstrated by abnormal cellular proliferation and proteoglycan-rich lesions detected by Alcian Blue staining and histological analysis.

Acknowledgments

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