ZFIN Image: Zhang et al., 2026, Fig. 4

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Figure Caption

Fig. 4

Negative impact of OGT knockdown in HEPM cells on in vitro osteogenic differentiation. a WB analysis of OGT protein level during osteogenic differentiation. b Representative Edu staining images of in shCtrl and shOGT HEPM cells. shCtrl short hairpin control (control lentivirus), shOGT short hairpin OGT (OGT lentivirus). c Expression of osteogenic markers (COL1A1, RUNX2, OSX, and OCN) and the protein levels of HEPM cells treated as indicated and assessed by WB. d Expressions of osteogenic genes (COL1A1, RUNX2, OSX, and OCN) and the mRNA levels of HEPM cells treated as indicated and assessed by RT-qPCR. e, f ALP and ARS staining of HEPM cells treated as indicated. Data were indicated by mean ± SD. n = 3 biologically independent experiments. P-values were computed by two-tailed t-test or one-way ANOVA followed by Tukey’s multiple comparisons test, *P < 0.05, **P < 0.01

Acknowledgments

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