PP1-related behavior changes against gradual intensity changes following sine waves. (A and B) Comparison of behavioral traces showing fish z-position changes and the amount of behavior change along the z-axis per second (ΔZ) in response to sine wave-followed short-wavelength light changes under a blue background, between PP1+/− (Top) and PP1−/− (Bottom) fish. (C) Power spectra showing periodicity in ΔZ through FFT analyses. (D and E) Comparative analyses of ΔZ before (Top) and after (Bottom) laser ablation of C-type tegmentum neurons (D) or sham operation (E). (F–K) Quantification of periodicities in ΔZ (F–H) and the correlation coefficients between sine wave-followed short-wavelength light intensity and smoothed-ΔZ (I–K). Comparison of these between PP1+/− and PP1−/− fish (F and I, Wilcoxon rank sum test), before and after laser ablation of C-type tegmentum neurons (G and J, paired t test), and after sham operation (H and K, paired t test). Experiments were performed using 10 to 11 dpf zebrafish larvae.
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