ZFIN Image: Wada et al., 2026, Fig. 1.

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Figure Caption

Fig. 1.

Light irradiation protocol generating PP1-specific responses in wild-type larvae. (A–C) Schematic representations illustrating the photoequilibrium between the two states of bistable PP1. (D) Calcium change profiles in PP1-expressing cells of Tg(pp1:GCaMP6s) larvae (5 to 6 dpf) in response to 405-nm (UV, purple bars) and 588-nm (visible light, yellow bars) laser stimuli, recorded using two-photon imaging. Bold and dotted traces indicate the mean and individual profiles, respectively (n = 5). Fluorescence intensities were normalized to the initial values. Spike-like calcium increases following visible-light exposures are due to apparatus lag time (transient darkness) during light-condition switching. (E) Averaged images (dorsal view) showing GCaMP fluorescence in PP1-expressing cells of the pineal organ in Tg(pp1:GCaMP6s) larvae over 50 frames. The images represent areas of 80 × 80 μm.

Acknowledgments

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