ZFIN Image: Chojnacki et al., 2026, Fig. 11

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Figure Caption

Fig. 11 In vivo click-to-release reaction of pyridine probes from tetrazylmethyl (TzMe) profluorophore. (a) Structure of the fluorogenic probe to monitor the removal of the TzMe group from pyridine by tert-butyl isonitrile (tBuNC) and formed fluorophore. (b) In vitro fluorescence turn-on upon reaction of TzMe-PyDPP with tBuNC (PBS + 2% DMSO, pH 7.6, 37 °C). (c) Zebrafish larvae (2 days post fertilization) were injected into the yolk sac with 2–3 nL TzMe-PyDPP (10 mM) as well as either an equivalent volume of tBuNC (50 mM) or DMSO (negative control) showing the enhanced fluorescence signal post-tBuNC injection. The images in each row represent the brightfield, detected fluorescence, and fluorescence overlaid on the brightfield. (d) Quantification of the fluorescence signal in larvae. Co-injected larvae displayed a 2.2-fold higher fluorescence intensity (t(14) = −10.93, p < 0.0001) than larvae injected with TzMe-PyDPP and DMSO. Comparison bars represent the standard error of the mean (SEM).

Acknowledgments

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