Figure 6

Figure 6

MFNG knockdown inhibits proliferation and metastatic capacity in colorectal cancer cells. (A) qRT-PCR analysis of MFNG expression in HCT-116 cells transduced with control shRNA or MFNG shRNA. (B) Western blot analysis and quantification of MFNG protein levels, confirming knockdown efficiency. (C) CCK-8 proliferation assay of HCT-116 cells with MFNG knockdown. (D) Wound healing assay showing migration of HCT-116 cells at 0 and 48 hours after scratch. (E) Transwell invasion assay of HCT-116 cells through Matrigel-coated membranes. (F) qRT-PCR analysis of MFNG expression in DLD-1 cells with control or MFNG knockdown. (G) Western blot analysis and quantification of MFNG protein expression. (H–J) CCK-8 proliferation (H), wound healing migration (I), and Transwell invasion (J) assays in DLD-1 cells with MFNG knockdown. Data represent mean ± SD from six independent experiments. **, p < 0.01; ***, p < 0.001.