Fig. 5.

Fig. 5.

Gene expression in aip^wh239 mutants exposed to PCB126 (experiment 3a). Heterozygous aip^wh239 zebrafish were crossed and their embryos were exposed in 96-well plates to DMSO (0.1%) or PCB126 (10 nM) from 6 to 24 hpf. At 72 hpf, larvae were genotyped and sampled for RNAseq. A) Multi-dimensional scaling (MDS) plot. B, left panel. Venn diagram showing the number of differentially expressed genes (DEGs) as a result of exposure to PCB126 in the 3 genotypes. B, right panel. Principle components analysis (PCA) plot of average normalized reads from each treatment group. C) Comparison of the fold-induction (up-regulation) of gene expression by PCB126 in HMZ aip mutant vs WT larvae. (CYP1A has been omitted from the correlation analysis). The linear regression (solid red line) is shown, along with the line of equal induction magnitude (1:1; dashed red line). D) Comparison of the fold down-regulation of gene expression by PCB126 in HMZ aip mutant vs WT larvae. The linear regression (solid blue line) is shown, along with the line of equal magnitude (1:1; dashed blue line). Additional information about this experiment can be found in Fig. S7. Abbreviations: PCB126: 3,3′,4,4′,5-pentachlorobiphenyl; WT: Wild-type; HET: Heterozygous; HMZ: Homozygous.