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Figure 5
Experimental timeline and analysis overview. Fertilized eggs were collected at approximately 2 h post-fertilization (hpf) and allowed to develop until 24 hpf. At this stage, the chorion was enzymatically removed using pronase. From 48 hpf, larvae were exposed for 72 h to 250 µM 6-OHDA, either alone or in combination with almond extract at concentrations of 5 or 25 µg/mL. Developmental parameters were monitored throughout the experimental period. At the end of the exposure, behavioural assessments, oxidative stress markers, and tyrosine hydroxylase (TH)-positive neurons (via immunofluorescence) were evaluated.