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Fig. 6 1,6-DHN activates the PPAR and P53 signaling pathways, inducing lipid accumulation and liver developmental defects.(A) The volcano plot illustrates the distribution of differentially expressed genes (DEGs) in zebrafish larvae treated with 1,6-DHN (0, 10 mg/L).(B)Enrichment analysis of differentially expressed metabolic function genes induced by 1,6-DHN, where the size of the dots represents the number of differentially expressed genes.(C)A network diagram of metabolic pathways and signaling pathways, showing the relationships between various metabolic and signaling pathways.(D-G) Molecular docking analysis of 1,6-DHN with CYP7A1 and P53 proteins. Panels D and E display the docking of 1,6-DHN with fish and human proteins regulated by CYP7A1, while panels F and G show the docking of 1,6-DHN with fish and human proteins regulated by P53.