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Fig. 5 1,6-DHN induces hepatocyte steatosis in larvae, leading to fat deposition.(A) Whole-body Oil Red O staining of larvae treated with different concentrations of 1,6-DHN.(B,C) Total cholesterol and triglyceride levels in larvae.(D) Heatmap of gene expression related to lipid metabolism pathways.(E) PAS staining of larvae exposed to 1,6-DHN.(F-G) mRNA levels of glycogen metabolism-related genes g6pca.1 and g6pca.2.Data are presented as mean ± SD from three independent experiments.(n = 20, *p < 0.05，**p < 0.01，***p < 0.001,mean ± S.D).Scale bar: 100 µm.
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