Fig. 4

Fig. 4

Cerebral artery diameter measurements after hyperglycemia in zebrafish larvae. (a-c) Procedure to quantify cerebral blood vessel diameter. (a) The zebrafish head was imaged using confocal microscopy. (b) Microscopical fluorescence images at 20x magnification were prepared and the cerebral blood vessel diameter was measured using ImageJ. (c) The diameter of middle mesencephalic central artery was determined in 2 branches at 4 sites (orange) on the 2 concentric circles (blue) drawn with the Concentric Circle plugin of ImageJ. Created in https://BioRender.com. (d) Quantification of the mesencephalic artery diameter. The diameter of the middle mesencephalic central artery in Tg(fli1:EGFP) zebrafish at 5 dpf increased after 48 h exposure to 60 mM and 130 mM glucose as compared to 0 mM glucose and mannitol conditions. An average of the diameter measured at 4 locations on the middle mesencephalic central artery was taken for n = 13–17 fish per group. Data are represented as mean ± SD. *P < 0.05, **P < 0.01.