Fig 4

Fig 4 Phenotypes of each fin in double and triple homozygous hox13 mutants.

(A) Whole-fish images of wildtype and triple hox13 mutant fish. Scale bars, 1 cm. (B-G) Caudal, dorsal, anal, pectoral and pelvic fin phenotypes for wildtype siblings, double homozygous, and triple homozygous mutants of interest. Replicates are listed as fractions for each genotype. The only inconsistency observed was joints appearing in the anal fins of mutants, as shown in S1C Fig. The genotypes of the fish are indicated on the left-hand side of the photos. Heterozygotes for each gene are shown in green, homozygous mutants are shown in red, and wildtypes are shown in black. Sample sizes are shown underneath the genotype of each fish. All fins of all genotypes showed high consistency. The only inconsistency was in the anal fin of the triple hox13 mutant, and the hoxa13a^-/-, hoxa13b^-/-, and hoxd13a^-/-, where sporadic, unorganized joints were observed in 3/10 and 2/8 replicates, respectively. Closeups images of these traits are also shown in Figs 1, S1, and S4. Scale bars, 2mm for caudal and anal fins, 1.5mm for dorsal fins, 1mm for pectoral fins, and 0.5mm for pelvic fins.