Figure 1

Figure 1

The wrn^-/- zebrafish exhibit short stature and undergo premature death

(A) Domain structures of the wild-type and mutant zebrafish Wrn protein. (Top) Wild-type Wrn protein contains exonuclease, helicase, RecQ C-terminal (RQC), helicase and RNaseD-like C-terminal (HRDC), and nuclear localization signal (NLS) domains. (Bottom) A frameshift mutation results in a truncation within the helicase domain.

(B) Survival curves of wrn^+/+ (black, n = 214), wrn^+/- (green, n = 472) and wrn^-/- (red, n = 133) zebrafish. Statistical significance was assessed using the log-rank test.

(C–F) Images of (C) wrn^+/+ (n = 9) and (D) wrn^-/- (n = 4) larvae at 21 dpf (scale bars, 1 mm), and (E) wrn^+/+ (n = 7) and (F) wrn^-/- (n = 5) juveniles at 75 dpf (scale bars, 5 mm).

(G) Body length measurements of wrn^+/+ larvae (n = 9) and wrn^-/- larvae (n = 4) at 21 dpf, and wrn^+/+ juveniles (n = 7) and wrn^-/- juveniles (n = 5) at 75 dpf. Statistical significance was assessed using an unpaired two-tailed Student’s t test. Data are presented as mean ± standard deviation (SD). The detailed mean ± SD are shown in Table S2.