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Figure 2

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ZDB-IMAGE-260215-9
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Peng et al., 2026 - Estrogen Receptor 2b Is Involved in Regulating Gonadotropin-Inhibitory Hormone Expression During Early Development in Zebrafish
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Figure 2

Construction of Tg (GnIH:mCherry) transgenic line. (A) The fragment used to drive mCherry fluorescence contains 2357 bp of 5′-flanking region and 50 bp of exon1. (B) The main elements of the plasmid used for generation of the transgenic zebrafish line, the plasmid contains tol2 elements and mCherry, the promoter elements were replaced by GnIH promoter fragments. (C) In situ hybridization of GnIH mRNA and mCherry fluorescence signal in transgenic zebrafish at 72 hpf (dorsal view).

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