Figure 7

Figure 7

Impairment of Vascular Function in Ciao3 Heterozygous Mice. A) H&E staining results of lungs of 9‐week‐old mice; the lungs of Ciao3^+/− mice were significantly thicker than those of wild‐type mice in terms of pulmonary artery wall and small blood vessel ratio. B) CD31 and α‐SMA dual fluorescence immunostaining in lungs of 9‐week‐old mice; results showed that CD31 expression was evenly distributed across the lung lobes of wild‐type mice; CD31‐positive cells were densely distributed, whereas α‐SMA expression was rarely distributed. C,D) The aortic rings of mice in matrix glue were cultured in ECM medium for 4 days. The results showed that the number of buds in aortic rings from Ciao3^+/−mice was significantly lower than in wild‐type mice. E, Illustrative example of mice movement in the open field test. F, Graphs showing the traveled distance, WT: 5268 ± 48 cm, n = 10; Ciao3^+/−: 6518 ± 76 cm, n = 10. G, Graphs showing the velocity, WT: 5.4 ± 0.54 cm/s, n = 10; Ciao3^+/−: 6.8 ± 0.63 cm/s, n = 10. H, Graphs showing the time in the center zone, WT: 162 ± 28s, n = 10; Ciao3^+/−: 219 ± 16s, n = 10. Data are represented as mean±SEM. Values were obtained by unpaired Student's t‐test, not significant; *p < 0.05; **p < 0.01; ***p < 0.001.