FIGURE 5

FIGURE 5

Nicotinic acid supplementation (partially) rescues survival and locomotor defects in naxd^−/− larvae. (A) Survival analysis of WT and naxd^−/− larvae without and with 100 μM nicotinic acid (B3) supplementation over 30 days (n = 30–50 larvae per genotype and condition). B3 supplementation was started at 7 dpf, and the water without or with the supplement was refreshed daily. (B) Locomotion behavior of untreated and B3‐supplemented WT and naxd^−/− larvae determined at 10 and 13 dpf. Scatter bar plots represent means ± SDs of the mean velocity calculated after continuous tracking for 30 min in light conditions (50% light intensity); each dot represents one larva (n = 10–12 per genotype and condition). (C–F) NAD(H), S‐ and R‐NADHX concentrations in extracts of 10 or 13 dpf larvae are shown as means ± SDs from three to four biological replicates, each replicate consisting of a batch of 10 whole larvae (raw data are provided in Table S3, experiment DOV33). Statistical significance was determined by one‐way ANOVA with multiple comparisons test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001; ns, not significant).