ZFIN Image: Gil et al., 2025, Figure 2

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Figure Caption

Figure 2

Mutation of hcfc1a does not increase response to PTZ. (A) Offspring of the hcfc1a^co60/+ allele were treated with 0.001 pM PTZ and assessed for behavioral patterns using the ZebraBox technology. A radar plot was used to compare the behavior of sibling wildtype (black) and heterozygous mutants (red). (B) Representative trace patterns were associated with behavior in (A). Three biological replicates were performed to achieve the total number of larvae, and panel (B) shows individual wells from each biological replicate. (C–F) Parameters from (A), for which there was a trending change in behavior, were individually analyzed using a bar graph and t-test. Small duration (C), small distance (D), large count (E), and total distance (F) are shown. Error bars represent the standard error of the mean. Sibling N = 19; hcfc1a^co60/+ N = 23. For representative trace patterns the color coding is consistent with the key in Figure 1.

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Phenotype details

Acknowledgments

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