Fig. 2

Fig. 2

AP2M1 is associated with the cell proliferation of the HCC cell line and embryonic hepatocytes. A HepG2 cells were transfected with AP2M1 siRNA for 72 h. The expression levels of AP2M1 were determined by western blotting using β-actin as an internal control. Data were quantified and expressed as the mean ± SEM of 5 independent experiments. ***p < 0.001 vs. value in the negative control. B HepG2 cell proliferation determined by an MTT assay was quantified and expressed as the mean ± SEM of 36 independent experiments. ***p < 0.001 vs. value in the negative control. C Hep3B cells were transfected with AP2M1 siRNA for 72 h. The expression levels of AP2M1 were determined by western blotting using β-actin as an internal control. Data were quantified and expressed as the mean ± SEM of 5 independent experiments. ***p < 0.001 vs. value in the negative control. D Hep3B cell proliferation determined by an MTT assay was quantified and expressed as the mean ± SEM of 36 independent experiments. ***p < 0.001 vs. value in the negative control. E HepG2 cell migration was determined by a wound-healing assay. Representative photomicrographs of cell monolayers at 48 h post-wounding. Cell migration area (%) was calculated from the ratio of changes in the wound area. Data were quantified and expressed as the mean ± SEM of 10 independent experiments. ***p < 0.001 vs. value in the negative control