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Fig. 5 DMH1 inhibition of Bmp signalling significantly decreased regenerating fin outgrowth, primarily along the dorsal and ventral margins of the amputated caudal fin. Similar levels and domains of GFP expression were detected in both DMSO (A,A′) and 10 μM DMH1 (E,E′) experimental groups of BRE1189 fish prior to treatment at 6dpa. (B-D′) The DMSO control group displayed consistent GFP expression throughout the six-day treatment period. At 8dpa (F,F′), following two days of DMH1 treatment, a decrease in GFP expression was observed, which became further reduced by 10dpa (G-G′), four days after DMH1 treatment. By 12dpa (H,H′), after six days of DMH1 treatment, the GFP signal in regenerating BRE1189 fins became nearly undetectable. Before treatment, GFP expression in amputated BRE510 caudal fins was similar in DMSO (I,I′) and DMH1 (K,K′) experimental groups at 6dpa. GFP expression remained similar in BRE510 fins at 12dpa, after six days of treatment with DMSO (J,J′) or DMH1 (L,L′). (M) Aggregate analysis of all rays revealed a significant reduction in outgrowth elongation in DMH1-treated fins compared to the control group treated with DMSO. (N) The most dorsal and ventral rays of amputated caudal fins had significantly decreased outgrowth when treated with DMH1, compared to DMSO controls, whereas the middle rays did not even show a trend of decreased outgrowth. Panels A′-L′ are higher-magnification views of regions identified by the yellow boxes in panels A–L, respectively, and the dashed white line represents the site of amputation. n = 3 for each genetic group for each chemical treatment at each timepoint. Scale bars: A-N = 250 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)