FIGURE 6

FIGURE 6

The arachidonic acid metabolism pathway was significantly altered in dKO embryos. (A) Heatmap of differentially expressed genes (DEGs) involved in the arachidonic acid metabolism pathway at 30, 48, and 72 hpf in dKO embryos compared to WT embryos. Upregulated genes (alox5b.1, alox5b.2, pla2g4c.1, alox5a) and downregulated genes (lta4h, ggt1a, gpx9) are shown. *p < 0.05. (B) Arachidonic acid is released by cytoplasmic phospholipase A2 (cPLA2s) and converted to 5-hydroperoxyeicosatetraenoic acid (5-HPETE) by 5LO. The pathway leads to the production of cysteinyl leukotriene (LTC4, LTD4, and LTE4), which signal via cysteinyl leukotriene receptors (CysLTRs). The cell region is highlighted with a light orange box. PM, plasma membrane. Cell and Cell’ denote two different cells. (C–E) Single-cell RNA-seq re-analysis shows the spatial and temporal expressions of 5LO (alox5a, alox5b.1, and alox5b.2) (C), cysltr1(D), and cysltr3(E). (C) At 19 and 24 hpf, the expression of 5LO homologs was enriched in hematopoietic progenitors, including blood islands and immune cells. At 48 and 72 hpf, expression remained prominent in immune cells and blood cells. (D)cysltr1 expression was highly enriched in the vasculature, including arteries, at 24 and 48 hpf. (E)cysltr3 showed its highest expression in the heart at 19 hpf but was not particularly enriched in the circulatory system.