ZFIN Image: Li et al., 2026, Figure 2.

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Figure Caption

Figure 2.

4-OHT–induced nuclear translocation of PGC-specific nCas9^ERT2in transgenic zebrafish embryos. (A) Schematics showing the mating strategy and 4-OHT treatment of embryos. (B) Immunostaining results for Cas9 (magenta) and Ddx4 (green) with nuclei stained by DAPI before and after 4-OHT treatment. The dashed boxes in the first row were enlarged and shown in the three lower rows. (C) Box plots showing the ratio of Cas9 (magenta) signal intensity in the nucleus to total PGCs (mean ± SD). Nu, nucleus. Each independent data point represents a PGC. The numbers of PGCs in control groups at 30% epi, shield, 75% epi, and bud stages are 10, 6, 4, and 10, respectively, while the numbers of PGCs in 4-OHT–treated groups at each stage are 4, 5, 11, and 10. P values of the t test (unpaired, two-sided) were also labeled. Source data are available for this figure: SourceData F2.

Acknowledgments

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