IMAGE

Figure 1

ID

ZDB-IMAGE-260101-70

Genes

mir499a

Source

Figures for Che et al., 2025

Image

Figure Caption

Figure 1

Generation and characterization of miR499 knockout. (A) Schematic illustration of miR499 mutation induction by the CRISPR/Cas9 system with two sgRNAs, and sequencing results of WT and miR499 mutant (F2) with 4—bp deletion (named miR499^−/−). Underlined and italicized text indicates the sgRNA target sequence. Green font denotes the pre-miRNA-499 sequence. (B) Semi-quantitative RT-PCR analysis of dre—miR499—5P and dre—miR499—3P expression in WT and miR499^−/− fish, with U6 as a loading control. *** p < 0.001. ns: no significance; (C) Representative images of WT and miR499^−/− fish at 30 days post-fertilization. Scale bars = 50 mm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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